| Identification | Back Directory | [Name]
DPN I | [CAS]
81295-14-9 | [Synonyms]
DPN I
DPN I RESTRICTION ENZYME
restriction endonuclease Dpn I
RESTRICTION ENDONUCLEASES DPN I
dpn i from diplococcus pneumoniae
restriction endonuclease dpn I from*diplococcus P
RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE | [MDL Number]
MFCD00166565 |
| Hazard Information | Back Directory | [Uses]
In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR. | [General Description]
Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis. |
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Sigma-Aldrich
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NEB (Beijing)
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www.neb-china.com |
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