| Identification | Back Directory | [Name]
EC 3.4.22.1 | [CAS]
9047-22-7 | [Synonyms]
EC 3.4.22.1
CATHEPSIN B
Cathepsin II
units/mg protein
Native Human Cathepsin B
Native Bovine Cathepsin B
CATHEPSIN B, BOVINE SPLEEN
CATHEPSIN B1, BOVINE SPLEEN
CATHEPSIN B FROM HUMAN LIVER
CATHEPSIN B FROM BOVINE SPLEEN
CATHEPSIN B FROM HUMAN PLACENTA
Cathepsin B from Human, recombinant
CathepsinB from human liver,CathepsinB1
CathepsinB from human placenta,CathepsinB1
CathepsinB from bovine spleen,Cathepsin B1 | [Molecular Formula]
NULL | [MDL Number]
MFCD00130748 |
| Hazard Information | Back Directory | [Uses]
Cathepsin B from human liver has been used:
- as a positive control in the screening Cathepsin B inhibitor assay
- in wall extension (expansin activity) assay
- in microinjection human foreskin fibroblasts to test its effect on apoptosis induction
| [Uses]
Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells. | [General Description]
Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal. | [Biochem/physiol Actions]
Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis. It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption. | [Purification Methods]
Cathepsin B is purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal-linked to Sepharose 4B, with elution by 2,2'-dipyridyl disulfide [Rich et al. Biochem J 235 731 1986, Methods Enzymol 80 551 1981]. |
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Sigma-Aldrich
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021-61415566 800-8193336 |
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https://www.sigmaaldrich.cn |
| Company Name: |
Energy Chemical
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021-58432009 400-005-6266 |
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http://www.energy-chemical.com |
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