494753-69-4

中文名称人参皂苷RK2

英文名称 Ginsenoside RK2

CAS 494753-69-4

分子式 C42H70O12

分子量 767

MOL 文件 494753-69-4.mol

更新日期 2024/05/27 17:16:47

494753-69-4 结构式

基本信息物理化学性质应用领域常见问题列表

基本信息

中文别名

人参皂苷RK1(RK1:RZ1=5:2)

英文别名

PAN 30
Ginsenoside RK2 USP/EP/BP
Ginsenoside Rk1 (Rk1:Rz1=5:2)
3beta,12beta-Dihydroxydammar-20(21),24-diene-3-O-beta-D-glucopyranosyl(1→
3β,12β-dihydroxydammar-20(21),24-diene-3-O-β-D-glucopyranosyl(1->2)-β-D-glucopyranoside
β-D-Glucopyranoside, (3β,12β)-12-hydroxydammara-20,24-dien-3-yl 2-O-β-D-glucopyranosyl-
3beta,12beta-Dihydroxydammar-20(21),24-diene-3-O-beta-D-glucopyranosyl(1-2)-beta-D-glucopyranoside

所属类别

生物化工：中草药成分

物理化学性质

外观性状白色粉末，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于人参。

沸点854.5±65.0 °C(Predicted)

密度1.27±0.1 g/cm3(Predicted)

储存条件Sealed in dry,2-8°C

溶解度Soluble in methan

酸度系数(pKa)12.85±0.70(Predicted)

形态粉末

应用领域

用途1

用于含量测定/鉴定/药理实验等。
药理药效:抗癌、提高免疫功能、调节神经系统。

用途2

人参皂苷Rk1是蒸参中提取的成分之一，具有抗肿瘤的生物活性。

常见问题列表

用途

Rk2一般通过水解人参皂苷二醇组获得，但水解成分复杂，不利于分离并准确鉴定结构。本文通过酶法水解高纯度的人参皂苷Rk2，水解产物通过高分辨质谱和核磁确证。

人参皂苷RK2结构式

功效与作用

人参皂苷Rk2能有效抑制肿瘤细胞生长，诱导分化癌细胞，具有抗浸润和抗转移疗效。

生物活性

Ginsenoside Rk1 人参皂苷 Rk1 是人参的高温加工提取物。 Ginsenoside Rk1 具有抗炎作用，抑制 Jak2/Stat3 信号通路和 NF-κB 的激活。 Ginsenoside Rk1 具有抗肿瘤作用，抗血小板聚集活性，抗炎作用，抗胰岛素抵抗，肾保护作用，抗菌作用，认知功能增强，脂质积聚减少和预防骨质疏松症。 Ginsenoside Rk1 通过触发细胞内活性氧 (ROS) 生成和阻断 PI3K/Akt 途径诱导细胞凋亡。

体外研究

Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation.

RT-PCR

Cell Line:	RAW264.7 cells
Concentration:	10 μM, 20 μM, 40 μM
Incubation Time:	6 hours
Result:	Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells.

Western Blot Analysis

Cell Line:	RAW264.7 cells
Concentration:	10 μM, 20 μM, 40 μM
Incubation Time:	6 hours
Result:	Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells

Cell Viability Assay

Cell Line:	MDA-MB-231 cells
Concentration:	0 μM, 40 μM, 80 μM, 120 μM
Incubation Time:	48 hours
Result:	Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner.

Cell Cycle Analysis

Cell Line:	MDA-MB-231 cells
Concentration:	0 μM, 40 μM, 80 μM, 120 μM
Incubation Time:	24 hours
Result:	Induced G0/G1 phase arrest.

Apoptosis Analysis

Cell Line:	MDA-MB-231 cells
Concentration:	0 μM, 40 μM, 80 μM, 120 μM
Incubation Time:	24 hours
Result:	Induced apoptosis in MDA-MB-231 cells.