86030-08-2
86030-08-2 结构式
基本信息
KIF18A抑制剂(BTB-1)
4-氯-2-硝基-1-(苯基磺酰基)苯
NSC156750
BTB 1
BTB1
LABOTEST-BB LT01147747
BTB 1 - NSC 156750 | NSC 658180
4-CHLORO-2-NITRODIPHENYL SULFONE
4-Chloro-2-nitrodiphenyl sulphone
4-CHLORO-2-NITRO-1-(PHENYLSULFONYL)BENZENE
1-Benzenesulfonyl-4-chloro-2-nitro-benzene
4-chloro-2-nitro-1-(phenylsulphonyl)benzene
物理化学性质
常见问题列表
| Target | Value |
|
Kif18A
(Cell-free assay) | 1.69 μM |
BTB-1 blocks the motility of Kif18A in a reversible manner. BTB-1 inhibits Kif18A in an adenosine triphosphate (ATP)-competitive but microtubule-uncompetitive manner and slows down the progression of cells through mitosis. 100 μM BTB-1 does not significantly inhibit any of the other tested mitotic kinesins. BTB-1 competes with ATP for Kif18A binding only when the motor-protein is associated with its pseudosubstrate microtubules. HeLa cells treated with BTB-1 accumulate in mitosis in a dose-dependent manner. BTB-1 shows cell toxicity with an EC 50 values of 35.8 μM. HeLa cells treated with 50 μM BTB-1 reveals severe defects in spindle morphology and chromosome alignment. Treatment with high concentrations of BTB-1 does not result in elongated spindles.