98849-88-8

中文名称 FLAG PEPTIDE

英文名称 FLAG peptide

CAS 98849-88-8

分子式 C41H60N10O20

分子量 1012.97

MOL 文件 98849-88-8.mol

更新日期 2024/06/02 19:54:13

98849-88-8 结构式

基本信息物理化学性质图谱信息常见问题列表

基本信息

中文别名

DDDDK肽
L-LYSINE, L-Α-ASPARTYL-L-TYROSYL-L-LYSYL-L-Α-ASPARTYL-L-Α-ASPARTYL-L-Α-ASPARTYL-L-Α-ASPARTYL-

英文别名

FLAG®
FLAG tag Peptide
FLAG PEPTIDE, >98%
FLAG Tag Peptide，FLAG epitope
FLAG PEPTIDE|DYKDDDDK|ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS
FLAG peptide,DYKDDDDK,Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, >98%
(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-amino-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]hexanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]hexanoic acid

所属类别

生物化工：多肽

物理化学性质

密度1.470±0.06 g/cm3 (20 ºC 760 Torr)

储存条件Keep in dark place,Sealed in dry,Store in freezer, under -20°C

溶解度≥50.65 mg/mL in DMSO; ≥210.6 mg/mL in H2O; ≥34.03 mg/mL in EtOH

形态冻干粉

序列H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH

InChIKeyXZWYTXMRWQJBGX-OVIGALBNNA-N

图谱信息

FLAG PEPTIDE(98849-88-8)质谱(MS)

常见问题列表

生物活性

FLAG peptide是一种八个氨基酸组成的多肽 (Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys)，具有肠激酶切割位点。FLAG peptide用于抗体介导的重组蛋白的识别和纯化。

体外研究

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca ²⁺ . Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca ²⁺ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.