4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide
![4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Structure](CAS/GIF/312756-74-4.gif)
- CAS No.
- 312756-74-4
- Chemical Name:
- 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide
- Synonyms
- RS-1;CS-2312;RS1;RS 1;RAD51-stimulatory compound 1;RAD51-Stimulatory Compound-1, RS-1;RS-1 - RAD51-Stimulatory Compound-1;RAD51,RS1,RS-1,inhibit,Inhibitor,RS 1,CRISPR/Cas9;3-benzylsulfamoyl-4-bromo-N-(4-bromophenyl)benzamide;3-(N-Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide;RAD51-Stimulatory Compound-1, RS-1 - CAS 312756-74-4 - Calbiochem
- CBNumber:
- CB02695334
- Molecular Formula:
- C20H16Br2N2O3S
- Molecular Weight:
- 524.23
- MDL Number:
- MFCD00348720
- MOL File:
- 312756-74-4.mol
- MSDS File:
- SDS
| Density | 1.680±0.06 g/cm3(Predicted) |
|---|---|
| storage temp. | room temp |
| solubility | DMSO: ≥10mg/mL |
| form | powder |
| pka | 10.16±0.30(Predicted) |
| color | off-white to light tan |
| Stability | Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months. |
| InChIKey | VBOWCZDVEOFGRY-UHFFFAOYSA-N |
| CAS DataBase Reference | 312756-74-4 |
4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide price More Price(27)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | R9782 | RS-1 ≥98% (HPLC) | 312756-74-4 | 5mg | $44.4 | 2024-03-01 | Buy |
| Sigma-Aldrich | 553510 | RAD51-Stimulatory Compound-1, RS-1 | 312756-74-4 | 10mg | $155 | 2024-03-01 | Buy |
| Cayman Chemical | 21037 | RS-1 ≥95% | 312756-74-4 | 5mg | $32 | 2024-03-01 | Buy |
| Cayman Chemical | 21037 | RS-1 ≥95% | 312756-74-4 | 10mg | $57 | 2024-03-01 | Buy |
| Sigma-Aldrich | R9782 | RS-1 ≥98% (HPLC) | 312756-74-4 | 25mg | $449 | 2024-03-01 | Buy |
4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Chemical Properties,Uses,Production
Description
RS-1 is an activator of DNA repair protein RAD51 (Kd = 48-107 for human RAD51 with different cofactors present). It stimulates homologous strand assimilation activity at least 5- to 11-old, enhancing homologous recombination activity of hRAD51. RS-1 is a potent enhancer of CRISPR-mediated genome editing, increasing homology directed repair 3- to 6-fold. It is used to increase CRISPR-mediated knock-in efficiencies in vitro and in vivo.
Uses
4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]benzamide is a stimulant of the human homologous recombination protein RAD51.
Uses
RS-1 has been shown to enhance CRISPR genome editing efficiency. To see other small molecule CRISPR enhancers, visit sigma.com/CRISPR-enhancers.
General Description
A cell-permeable sulfonamido-benzamide-based allosteric regulator that stimulates DNA binding and recombinase activities of hRAD51 by locking hRAD51 in an active conformation without affecting its active site ATP hydrolysis. Although RS-1 enhances hRAD51 filament formation on ssDNA with or without the cofactor NTP, active filaments and recombinase activity are only induced in the presence of ATP or AMP-PNP, but not with ADP or no cofactors. Shown to promote resistance of primary human neonatal dermal fibroblasts to Cisplatin- (Cat. No. 232120) induced death in a dose-dependent manner. RS-1 is inactive toward related DNA strand exchange proteins scRAD5 and scDMC1 of yeast origin or E. coli RedA.This HDR (homology-directed repair) enhancer, is shown to significantly increase both Cas9 & TALEN-mediated knock-in efficiencies.
Biological Activity
RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer that enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.
Biochem/physiol Actions
RS-1 is a sulfonamido-benzamide compound.
in vitro
RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity[1]. RS-1 enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.
storage
Room temperature
References
1) Jayathilaka et al. (2008), A chemical compound that stimulates the human homologous recombination protein RAD51; Proc. Natl. Acad. Sci. USA 105 15848 2) Mason et al. (2014), The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors; Cancer Res. 74 3546 3) Pinder et al. (2015), Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing; Nucleic Acids Res. 43 9379 4) Song et al. (2016), RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency; Nat. Commun. 7 10548
4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| ATK CHEMICAL COMPANY LIMITED | +undefined-21-51877795 | ivan@atkchemical.com | China | 32715 | 60 |
| career henan chemical co | +86-0371-86658258 15093356674; | factory@coreychem.com | China | 29826 | 58 |
| Zhejiang J&C Biological Technology Co.,Limited | +1-2135480471 +1-2135480471 | sales@sarms4muscle.com | China | 10523 | 58 |
| InvivoChem | +1-708-310-1919 +1-13798911105 | sales@invivochem.cn | United States | 6393 | 58 |
| Nantong HI-FUTURE Biology Co., Ltd. | +undefined18051384581 | sales@chemhifuture.com | China | 3136 | 58 |
| ShenZhen Trendseen Biological Technology Co.,Ltd. | 13417589054 | trendseenbio@gmail.com | China | 11681 | 58 |
| LEAPCHEM CO., LTD. | +86-852-30606658 | market18@leapchem.com | China | 43348 | 58 |
| Aladdin Scientific | +1-+1(833)-552-7181 | sales@aladdinsci.com | United States | 57511 | 58 |
| Amadis Chemical Company Limited | 571-89925085 | sales@amadischem.com | China | 131980 | 58 |
| MQ (shanghai) Pharmaceuticals Co., Ltd. | 13761635123 | 1014988033@qq.com | China | 4986 | 55 |
View Lastest Price from 4-BroMo-N-(4-broMophenyl)-3-[[(phenylMethyl)aMino]sulfonyl]benzaMide manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2020-01-02 | 3-(Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide
312756-74-4
|
US $1.00 / KG | 1KG | Min98% HPLC | g/kg/ton | Career Henan Chemical Co |
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- 3-(Benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide
312756-74-4
- US $1.00 / KG
- Min98% HPLC
- Career Henan Chemical Co
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