NNMTi
- CAS No.
- 42464-96-0
- Chemical Name:
- NNMTi
- Synonyms
- 5-amino-1MQ;NNMTi;NNMTi 5-amino-1MQ;5-amino-1MQ/5-amino-1mq;5-Amino-1-methylquinolinium;5-Amino-1methylquinolinium iodide;5-amino-1-methylquinolin-1-ium iodide;5-Amino-1-methyl-1-quinolinium Iodide;1-methylquinolin-1-ium-5-amine iodide;NNMTi,inhibit,fusion,muSC,muscle stem cell,NNMT,Inhibitor,regenerative
- CBNumber:
- CB05839892
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
SAFETY
Risk and Safety Statements
Symbol(GHS) | GHS07 |
---|---|
Signal word | Warning |
Hazard statements | H302-H315-H319-H335 |
Precautionary statements | P261-P264-P270-P271-P280-P301+P312-P302+P352-P304+P340-P305+P351+P338-P312-P330-P332+P313-P337+P313-P362-P403+P233-P405-P501 |
NNMTi Chemical Properties,Uses,Production
Description
NNMTi (CAS 42464-96-0) is a nicotinamide N-methyltransferase (NNMT) inhibitor that promotes myoblast differentiation. NNMT is a fundamental cytosolic biotransforming enzyme that catalyzes the N-methylation of endogenous and exogenous xenobiotics. NNMTi treatment combined with lean diet substitution accelerated improved body weight fat loss, increased whole-body lean mass to body weight ratio, reduced liver epididymal white adipose tissue weights, decreased liver adiposity, improved hepatic steatosis, relative to a lean diet substitution alone.
Chemical Properties
Brown solid.
Uses
NNMTi is an enzyme overexpressed during skeletal muscle aging and is associated with the repair of damage to the NAD+ pathway, dysregulation of sirtuin1 activity, and increased senescence of muscle stem cells.
Preparation
Synthesis of 5-Amino-1-methyl-1-quinolinium Iodide(NNMTi): Iodomethane (0.160 ml, 0.365 g, 2.6 mmol) was added to a solution of 5-aminoquinoline (0.277 g, 1.9 mmol) in tetrahydrofuran (7 ml), and stirred at room temperature for 1 day until a red solid precipitated. After filtration, the filter cake was washed with tetrahydrofuran (2 x 5 ml) and dried under vacuum to give 0.240 g of compound 5-Amino-1-methyl-1-quinolinium Iodide.
Biological Activity
NNMTi is a potent nicotinamide N-methyltransferase (NNMT) inhibitor (IC50=1.2 μM), selectively binding to NNMT substrate binding site residues. NNMTi can promote myoblast differentiation in vitro and enhance the fusion and regeneration of muscle stem cells in aged mice.
in vitro
NNMTi (10-30 μM; 96 hours) produces a concentration-related increase in myoblast differentiation on C2C12 myoblast differentiation. 30 μM NNMTi results in 18% MHC-positive myotube nuclei, representing a 45% increase in the extent of myoblast differentiation compared to untreated differentiating myoblasts (12% MHC-positive myotube nuclei).
in vivo
NNMTi (subcutaneous (SC) injection; 5 mg/kg and 10 mg/kg; 2 weeks (1 week pre-injury and 1 week post-injury)) has an effect on muscle regeneration after injury, it results in 60% and 75% higher incidence of proliferating/active muSCs at 5 mg/kg and 10 mg/kg, respectively. The relative numbers of fibers with an EdU + myonucleus increased 40% and 48% with NNMTi treatment at 5 mg/kg and 10 mg/kg, respectively. The odds ratio of fused myonuclei for control are 0.58 and 0.53 times the odds at the low and high NNMTi dose, respectively.NNMTi (subcutaneous injection; 10 mg/kg; 1 week) produces no systemic toxicity in mice, the levels of the glucose, cholesterol, plasma proteins, and electrolytes between control and NNMTi-treated samples show no difference in mice. 1-week post-injury daily repeat-dosing of NNMTi is well tolerated with no untoward systemic toxicity or behavioral implications in aged mice.
storage
Store at -20°C
References
[1]. Harshini Neelakantan, et al. Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle. Biochem Pharmacol. 2019 May;163:481-492. DOI:10.1016/j.bcp.2019.02.008
[2]. Harshini Neelakantan, et al. Structure-Activity Relationship for Small Molecule Inhibitors of Nicotinamide N-Methyltransferase. J Med Chem DOI:10.1021/acs.jmedchem.7b00389
[3]. Mark A Eckert et al. Proteomics reveals NNMT as a master metabolic regulator of cancer-associated fibroblasts. Nature, 569(7758), 723-728 (2019-05-03) DOI:10.1038/s41586-019-1173-8
[4]. SAMPSONCATHERINE M. Combined nicotinamide N-methyltransferase inhibition and reduced-calorie diet normalizes body composition and enhances metabolic benefits in obese mice.[J]. Scientific Reports, 2021: 5637. DOI:10.1038/s41598-021-85051-6.
NNMTi Preparation Products And Raw materials
NNMTi Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Zhejiang Hangyu API Co., Ltd | +8617531972939 | anna@api-made.com | China | 2944 | 58 |
Shanghai Affida new material science and technology center | +undefined15081010295 | admin@oudaxin.com | China | 371 | 58 |
Hebei Kangcang new material Technology Co., LTD | +8615713292910 | Nancy@kangcang.com.cn | China | 341 | 58 |
CONTIDE BIOTECH CO.,LTD | +852-53358525 | xena@healthtide-api.com | China | 619 | 58 |
Hebei Zhuanglai Chemical Trading Co.,Ltd | +8613343047651 | admin@zlchemi.com | China | 2196 | 58 |
Zhejiang J&C Biological Technology Co.,Limited | +1-2135480471 +1-2135480471 | sales@sarms4muscle.com | China | 10523 | 58 |
Henan Alfa Chemical Co., Ltd | +8618339805032 | alfa4@alfachem.cn | China | 12975 | 58 |
Jiangsu shring Biopharma Co., Ltd. | +8613372282299 | sales@shringchem.com | China | 3545 | 58 |
Hangzhou MolCore BioPharmatech Co.,Ltd. | +86-057181025280; +8617767106207 | sales@molcore.com | China | 49739 | 58 |
Hebei Nafu Technology Co. , Ltd. | +8615075022224 | 17745771666@hebeinafu.com | China | 325 | 58 |
View Lastest Price from NNMTi manufacturers
Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
---|---|---|---|---|---|---|---|---|
2024-05-27 | NNMTi
42464-96-0
|
US $70.00-65.00 / box | 1box | 99.9+ | 2000box | Strong peptide cross-border e-commerce Co. LTD | ||
2024-05-27 | NNMTi 5-amino-1MQ
42464-96-0
|
US $0.00-0.00 / kg | 1kg | 99% | 500 | Wuhan Haorong Biotechnology Co.,Ltd | ||
2024-05-27 | 5-amino-1mq
42464-96-0
|
US $0.00 / G | 1G | 99% | 5kg | CONTIDE BIOTECH CO.,LTD |
- NNMTi
42464-96-0
- US $70.00-65.00 / box
- 99.9+
- Strong peptide cross-border e-commerce Co. LTD
- NNMTi 5-amino-1MQ
42464-96-0
- US $0.00-0.00 / kg
- 99%
- Wuhan Haorong Biotechnology Co.,Ltd
- 5-amino-1mq
42464-96-0
- US $0.00 / G
- 99%
- CONTIDE BIOTECH CO.,LTD