LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI O55:B5

Uses

FITC-Lipopolysaccharides (FITC-LPS) may be used to study the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems. FITC-LPS may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures. FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.

Uses

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Uses

Lipopolysaccharide from Escherichia coli O55:B5 has been used:

• to stimulate hepatocytes and non-parenchymal cells
• to stimulate the spleen and study the expression of the Malitaf gene in the fish blunt snout bream
• as part of the concanavalin A (ConA) and D-galactosamine/lipopolysaccharide (D-GalN/LPS) model of fulminant hepatitis to study the effect of the natural compound hesperetin

General Description

Lipopolysaccharides (LPSs) consist of a lipid A moiety linked to an antigenic O-polysaccharide.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.