レクチン バンデイラエアシンプリシフォリア(BANDEIRAEA SIMPLICIFOLIA(GRIFFONIA SIMPLICIFOLIA))由来 化学特性,用途語,生産方法
使用
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
一般的な説明
Lectin from
Bandeiraea simplicifolia (
Griffonia simplicifolia) has A and B subunits, which recognizes αGalNAc and αGal-end groups respectively. Lectins are extracted from the seeds of the legume shrub
Griffonia simplicifolia, which are carbohydrate binding proteins.
Biochem/physiol Actions
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
レクチン バンデイラエアシンプリシフォリア(BANDEIRAEA SIMPLICIFOLIA(GRIFFONIA SIMPLICIFOLIA))由来 上流と下流の製品情報
原材料
準備製品