T4 DNAリガーゼ 化学特性,用途語,生産方法
用途
NAとRNAのライゲーションや完全長cDNAのクローニング。
使用上の注意
本品を自動霜取り機がついた氷冷機には保存しない
使用
Forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. For ligation of cloning vector and restriction insert fragments
一般的な説明
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.
ContentsT4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.
T4 DNAリガーゼ 上流と下流の製品情報
原材料
準備製品