ANTI-NEOMYCIN PHOSPHOTRANSFERASE II 化学特性,用途語,生産方法
使用
Anti-NPTII antibody has been used in immunoblotting.
一般的な説明
Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from the
E. coli transposon Tn5.
Biochem/physiol Actions
The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.
ANTI-NEOMYCIN PHOSPHOTRANSFERASE II 上流と下流の製品情報
原材料
準備製品