ホスファチジルエタノールアミン 化学特性,用途語,生産方法
外観
澄明の液体
定義
本品は、コムギ胚芽油(*)から得られるホスファチジルエタノールアミンである。
RCO：コムギ胚芽油から得られた脂肪酸アシル基
参照表示名称：コムギ胚芽油
溶解性
水に難溶 (0.8g/100ml水, 20℃), アルコール, ベンゼンと混和。水に溶けにくい。
解説
ホスファチジルエタノールアミン，グラム陰性細菌の主要リン脂質で，大腸菌では全リン脂質の65～85％ を占める．生物体からの抽出精製法は多数知られているが，代表的なものはクロロホルム-無水エタノールによる抽出，分画法で，抽出物からはシリカゲルやDEAE-セルロースカラムクロマトグラフィーにより，容易に精製することができる．
性質
ホスファチジルエタノールアミン，弱酸性のリン脂質で，ほかのものに比べて親水性に乏しく，水に懸濁しにくい．エタノール，メタノール，エーテル，石油エーテル，クロロホルム，ベンゼンに可溶，アセトンに不溶．合成されたジステアロイル-L-α-グリセリルホスホリルエタノールアミンは白色の粉末．融点175 ℃．ホスホリパーゼAの作用により，脂肪酸が1個除去されたリゾホスファチジルエタノールアミンを生じる．生体内では，ホスファチジルセリンの脱炭酸またはジグリセリドとCDP-エタノールアミンとの反応により生合成される．
用途
りん脂質代謝研究、生体膜研究。
用途
りん脂質の代謝研究，生体膜研究。
化粧品の成分用途
皮膚コンディショニング剤
使用
L-α-Phosphatidylethanolamine from egg yolk has been used:
- as a component of lipid mixture for the synthesis of giant unilamellar vesicles (GUVs)
- in ceramide phosphoethanolamine (CPE) synthase assay using crude sphingomyelin synthase-related protein from Hela cells
- for the standard curve generation for the quantification of lipids in plantaris muscle homogenates from mice
一般的な説明
L-α-Phosphatidylethanolamine from egg yolk has high unsaturated fatty acid content and elicits radical-scavenging activity. L-α-Phosphatidylethanolamine (PE) is a membrane phospholipid and is interconverted to phosphotidylserine in the presence of phosphatidylserine synthase 2. PE is essential for maintaining the structural integrity of membranes. It is the second most abundant phospholipid in animals, plants and yeast and the major lipid in bacteria. It is synthesized in mitochondria in mammals.
酵素阻害剤
This hygroscopic phospholipid, also called cephalin and 1,2-diacyl-snglycero- 3-phosphoethanolamine, is an important constituent of biomembranes and lipoproteins. It is the major structural phospholipid in brain tissue. The physical properties depend on the nature of the acyl groups and on the presence of other lipids. Freshly prepared phosphatidylethanolamines are white solids that turn yellow and brown on exposure to air. They typically sinter between 80 and 90°C and melt in the neighborhood of 175°C. Solutions of phosphatidylethanolamine slowly decompose at room temperature (roughly 0.3-0.5% per day); hence, solutions should always be freshly prepared and kept cold prior to use. It is labile in alkaline conditions. See also specific compound Target(s): b- N-acetylglucosaminyl-glycopeptide b-1,4-galactosyltransferase; Nacetyllactosamine synthase; acylglycerol kinase, or monoacylglycerol kinase; cholesterol monooxygenase, side-chain-cleaving, or CYP11A1; chymase; cytidylate cyclase; dolichyldiphosphatase; dolichyl-phosphatase; dopamine b-monooxygenase; ethanolaminephosphotransferase; glutamate dehydrogenase; γ-glutamyl transpeptidase, mildly inhibited; glycerol-3-phosphate Oacyltransferase; glycerone-phosphate O-acyltransferase, or dihydroxyacetione-phosphate O-acyltransferase; hormone-sensitive lipase; 3-hydroxybutyrate dehydrogenase; indole-3-acetate b-glucosyltransferase; phosphatidate cytidylyltransferase; 1- phosphatidylinositol 4-kinase; phosphoinositide phospholipase C; phospholipase D, phosphatidylcholine-specific enzyme; sphingomyelin phosphodiesterase; steryl-b-glucosidase; UDP-Nacetylglucosamine: dolichyl-phosphate N-acetylglucosaminephosphotransferase.
純化方法
Purify the cephalin by dissolving it in EtOH, adding Pb(OAc)2.3H3O (30g in 100mL H2O) until excess Pb2+ is present. Filter off the solid. Pass CO2 gas through the solution until precipitation of PbCO3 ceases. Filter the solid off and evaporate (while bubbling CO2) under vacuum. An equal volume of H2O is added to the residual oil and extracted with hexane. The hexane extract is washed with H2O until the aqueous phase is free from Pb [test with dithizone (2 mg in 100 mL CCl4; Feigel Spot Tests Vol I, Elsevier p. 10 1954)]. The hexane is dried (Na2SO4), filtered and evaporated to give a yellow waxy solid which should be dried to constant weight in vacuo. It is practically insoluble in H2O and Me2CO, but freely soluble in CHCl3 (5%) and Et2O, and slightly soluble in EtOH. [Schofield & Dutton Biochemical Preparations 5 5 1957.]
ホスファチジルエタノールアミン 上流と下流の製品情報
原材料
準備製品