| 9028-00-6 Basic information More.. |
Product Name: | CLOSTRIPAIN | Synonyms: | ENDOPROTEINASE-ARG-C;EC 3.4.22.8;CLOSTRIPAIN;CLOSTRIDIOPEPTIDASE B;IUB: 3.4.22.8;PROTEINASE;clostripain from clost. histolyticum;CLOSTRIPAIN FROM CLOST. HISTOLYTICUM >1 | CAS: | 9028-00-6 | MF: | | MW: | 0 | EINECS: | 232-822-2 | Mol File: | Mol File | |
Use
Clostripain is isolated from Clostridium histolyticum callogenase by extraction in pH 6.7 buffer, followed by hydroxylapatite chromatography with a 0.1-0.2 M phosphate gradient, then Sephadex G-75 gel filtration with 0.05M phosphate pH 6.7, dialysis and a second hydroxylapatite chromatography (gradient elution with 0.1M 0.3M phosphate, pH 6.7). It has proteinase and esterase activity and is assayed by hydrolysing N-benzoyl-L-arginine methyl ester. [Mitchell & Harrington J Biol Chem 243 4683 1968, Methods Enzymol 19 635 1970.]
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9028-00-6
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