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| | BCECF-AM Basic information |
| Product Name: | BCECF-AM | | Synonyms: | 2',7'-BIS-(2-CARBOXYETHYL)-5-(AND-6)-CARBOXYFLUORESCEIN, ACETOXYMETHYL ESTER;2,7-BIS(2-CARBOXYETHYL)-5,6-CARBOXYFLUORESCEIN;2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER;2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN ACETOXYMETHYL ESTER;2',7'-bis(2-carboxyethyl)-5(6)-*carboxyfluorescei;BCECF.AM ESTER, 1 MG/ML IN*ANHYDROUS DMSO;Bcecf-AmSolution;2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester mixed isomers | | CAS: | 117464-70-7 | | MF: | C42H40O21 | | MW: | 880.75 | | EINECS: | | | Product Categories: | | | Mol File: | 117464-70-7.mol |  |
| | BCECF-AM Chemical Properties |
| Boiling point | 692.41°C (rough estimate) | | density | 1.2837 (rough estimate) | | refractive index | 1.6500 (estimate) | | Fp | 85 °C | | storage temp. | -20°C | | form | White to off-white solid | | CAS DataBase Reference | 117464-70-7(CAS DataBase Reference) |
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ACROS
| English |
| | BCECF-AM Usage And Synthesis |
| Description | BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours. Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF AM can also be used to investigate intracellular changes in other ions, including potassium. | | Chemical Properties | pale yellow to yellow powder | | Uses | Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells. | | General Description | Material is an isomeric mixture of lactones, acetates, and AM esters | | Biological Activity | Non-charged, non-fluorescent pH indicator. Readily and rapidly diffuses through cell membranes. |
| | BCECF-AM Preparation Products And Raw materials |
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