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Postion:Product Catalog >Dyes and Pigments>dye>Acid dye> Sulforhodamine B
Sulforhodamine B
  • Sulforhodamine B
  • Sulforhodamine B

Sulforhodamine B NEW

Price $6 $5 $1
Package 1KG 25KG 100KG
Min. Order: 1KG
Supply Ability: g-kg-tons, free sample is available
Update Time: 2024-03-29

Product Details

Product Name: Sulforhodamine B CAS No.: 3520-42-1
Min. Order: 1KG Purity: 99%
Supply Ability: g-kg-tons, free sample is available Release date: 2024/03/29
Lead time: In stock Packaging: bottle/drum/bucket/IBC, as request.
Delivery: By sea, by air, by express Origin: Manufacturer
Name: Sun

1. Product information

Names

Namelissamine rhodamine
SynonymMore Synonyms

 Acid Red 52 Biological Activity

DescriptionSulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
Related Catalog
Signaling Pathways >> Others >> Others
Dye Reagents
Research Areas >> Others
In VitroSulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1].
Cell AssayGently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1].
References

[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

 Chemical & Physical Properties

Molecular FormulaC27H29N2NaO7S2
Molecular Weight580.65
PSA150.78000
LogP7.81310
Water Solubilitymethanol: 1 mg/mL

 MSDS

Acid Red 52 MSDS(Chinese)

 Toxicological Information

CHEMICAL IDENTIFICATION

  • RTECS NUMBER :

  • BP6750000

  • CHEMICAL NAME :

  • Ammonium, (6-(diethylamino)-9-(2,4-disulfophenyl)-3H-xanthen-3- ylidene)diethyl-, hydroxide, inner salt, sodium salt

  • CAS REGISTRY NUMBER :

  • 3520-42-1

  • LAST UPDATED :

  • 199701

  • DATA ITEMS CITED :

  • 5

  • MOLECULAR FORMULA :

  • C27-H29-N2-O7-S2.Na

  • MOLECULAR WEIGHT :

  • 580.69

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

  • TYPE OF TEST :

  • LD50 - Lethal dose, 50 percent kill

  • ROUTE OF EXPOSURE :

  • Oral

  • SPECIES OBSERVED :

  • Rodent - mouse

  • DOSE/DURATION :

  • 10300 mg/kg

  • TOXIC EFFECTS :

  • Details of toxic effects not reported other than lethal dose value

MUTATION DATA

  • TYPE OF TEST :

  • Cytogenetic analysis

  • TEST SYSTEM :

  • Rodent - hamster Fibroblast

  • DOSE/DURATION :

  • 13100 mg/L

  • REFERENCE :

  • FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House, Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year: 22,623,1984 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M2208 No. of Facilities: 114 (estimated) No. of Industries: 5 No. of Occupations: 7 No. of Employees: 901 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M2208 No. of Facilities: 2248 (estimated) No. of Industries: 47 No. of Occupations: 51 No. of Employees: 75084 (estimated) No. of Female Employees: 12598 (estimated)

 Safety Information

SymbolArticle illustration
GHS07
Signal WordWarning
Hazard StatementsH315-H319-H335
Precautionary StatementsP261-P305 + P351 + P338
Personal Protective EquipmentEyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard CodesXi
Risk Phrases36/37/38
Safety Phrases22-24/25
RIDADRNONH for all modes of transport
WGK Germany2
RTECSBP6750000
HS Code3204120000

 Precursor & DownStream

Precursor  0



DownStream  1



  • Article illustration CAS#:62796-29-6
    lissamine rhoda...

 Customs

HS Code2921499090
Summary2921499090 other aromatic monoamines and their derivatives; salts thereof VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:6.5% General tariff:30.0%

 Articles180

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Enhancing the anti-inflammatory activity of chalcones by tuning the Michael acceptor site.

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 Synonyms

4'-Butoxy-3-piperidinopropiophenone hydrochloride
Tanaclone
EINECS 222-529-8
dyclonine hydrochloride
SULFORHODAMINE B
MFCD00010180
C.I. 45100
Xanthylium, 3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-, inner salt, sodium salt (1:1)
dye acid red 52
Sodium 4-[6-(diethylamino)-3-(diethyliminio)-3H-xanthen-9-yl]benzene-1,3-disulfonate
Sulforhodamine-B
Dyclone
N,N,N',N'-tetraethylsulforhodamine
rhodamine 200 S sodium salt
Dyclocaine hydrochloride
Dyclonine HCL
Acid Red 52,Sulforhodamine B monosodium salt
Dyclothane
Acid Red 52
C.I.Acid Red 52
Sulforhodamine B sodium salt
Sulforhodamine B (sodium salt)


2. Packaging

For powders: normal is 25kgs/Drum or bag, or larger/smaller package as request.

For liquids: normal 25kgs/drum, 180-300kgs/bucket, or IBC, determined by the nature of the product. 

                     Or smaller package 1kg/bottle, 10kgs/bottle as request. 


Article illustrationArticle illustration


3. Shipping

Article illustration


4. Contact information

For more details, pls contact us freely.

Email address : Sun@fdachem.com

Mob: 86 13526505137

WhatsApp/Skype/Wechat/LINE: 86 13526505137





Company Profile Introduction

Henan Fengda Chemical Co., Ltd. is located in the High-tech Development Zone of Henan Province. Specializing in the production and sales of various fine chemical products required for industrial production, including chemical raw materials, organic raw materials, petrochemicals, chemical reagents, solvents, catalysts, and additives, etc.

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Henan Fengda Chemical Co., Ltd

1YR ChinaChina
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  • Since: 2023-02-10
  • Address: Room 01, 2288 E05, Building 14, East Henan University, Science and Technology Park, 279 Xisanhuan Ro
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