| Hazard Information | Back Directory | [Definition]
ChEBI:Phosphoramide mustard is a nitrogen mustard and a phosphorodiamide. | [Biological Activity]
Phosphoramide mustard is the active metabolite of cyclophosphamide (HY-17420) with antitumor activity. It induces DNA damage. | [in vitro]
Phosphoramide mustard causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication.
It (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells ( SIGCs).
Phosphoramide mustard (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage.
It (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins.
Cell Viability Assay | Cell Line: | SIGCs | | Concentration: | 0.5 μM, 1 μM, 3 μM, 6 μM | | Incubation Time: | 48 hours | | Result: | Reduced ce ll viability at concentrations of 3 μM and higher. | RT-PCR | Cell Line: | SIGCs | | Concentration: | 3 μM, 6 μM | | Incubation Time: | 24 hours, 48 hours | | Result: | Increased DDR gene mRNA expression levels. | Western Blot Analysis | Cell Line: | SIGCs | < /tr> | Concentration: | 3 μM, 6 μM | | Incubation Time: | 24 hours, 48 hours | | Result: | Generally increased DDR pro teins. | | [target]
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