| Identification | Back Directory | [Name]
Biotinylated isoxazole 95% | [CAS]
1377605-22-5 | [Synonyms]
Biotin isoxazole
Biotinylated isoxazole
Biotinylated isoxazole 95%
1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, 6-[[[5-(2-thienyl)-3-isoxazolyl]carbonyl]amino]hexyl ester, (3aS,4S,6aR)- | [Molecular Formula]
C24H32N4O5S2 | [MOL File]
1377605-22-5.mol | [Molecular Weight]
520.66 |
| Chemical Properties | Back Directory | [Boiling point ]
800.4±65.0 °C(Predicted) | [density ]
1.251±0.06 g/cm3(Predicted) | [storage temp. ]
-20°C | [solubility ]
Soluble in DMSO (30 mg/ml with warming) | [form ]
powder or crystals | [pka]
13.77±0.46(Predicted) | [color ]
Beige | [Stability:]
Stable for 2 years as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month. |
| Hazard Information | Back Directory | [Description]
Biotin-isoxazole (1377605-22-5) precipitates hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules.1,2 In one study roughly 580 nuclear proteins were identified as being precipitated by B-Isox including TAF15, RNA polymerase II (largest subunit), numerous subunits of the mediator complex as well as a variety of enzymes involved in epigenetic modification of histones.3? SR domains were found to be the determinant that facilitates B-Isox precipitation.4 Also allows for the fractionation / isolation of SG-associated proteins and RNA precipitates.5 | [Uses]
When exposed to cells or tissue lysates, biotinylated isoxazole (b-isox) was shown to precipitate RNA-binding proteins with significant overlap to the components of RNA granules. By reversibly aggregating and disaggregating structures similar to RNA granules, b-isox has lent insight to polypeptides necessary for the reversible aggregation of RNA-binding proteins.
This product was previously listed as T511617. | [References]
Kato et al. (2012), Cell-free formation of rNA granules: low complexity sequence domains form dynamic fibers within hydrogels; Cell, 149 753
Han et al. (2012), Cell-free formation of RNA granules: bound RNAs identify features and components of cellular assemblies; Cell, 149 768
Kwon et al. (2013), Phosphorylation-regulated binding of RNA polymerase II to fibrous polymers of low-complexity domains; Cell, 155 1049
Kwon et al. (2014), Poly-dipeptides encoded by the C9orf72 repeats bind nucleoli, impede RNA biogenesis, and kill cells; Science, 345 1139
Panas et al. (2015), Methods for the characterization of stress granules in virus infected cells; Methods, 90 57 |
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