| Identification | Back Directory | [Name]
AZ-27 | [CAS]
1516772-44-3 | [Synonyms]
AZ-27
4H-Thieno[3,2-d][1]benzazepine-2-carboxamide, N-cyclopropyl-5,6-dihydro-6-[4-[[[2-(2-oxa-7-azaspiro[3.5]non-7-yl)-3-pyridinyl]carbonyl]amino]benzoyl]- | [Molecular Formula]
C36H35N5O4S | [MOL File]
1516772-44-3.mol | [Molecular Weight]
633.76 |
| Hazard Information | Back Directory | [Uses]
N-Cyclopropyl-5,6-dihydro-6-[4-[[[2-(2-oxa-7-azaspiro[3.5]non-7-yl)-3-pyridinyl]carbonyl]amino]benzoyl]-4H-thieno[3,2-d][1]benzazepine-2-carboxamide is a possible respiratory syncytial virus (RSV) RNA polymerase inhibitor. | [Enzyme inhibitor]
This RSV inhibitor (FW = 633.77 g/mol) targets Respiratory Syncytial Virus, inhibiting both mRNA transcription and genome replication in cell based mini-genome assays, suggesting it targets a step common to both of these RNA synthesis processes. Analysis in an in vitro transcription run-on assay, containing RSV nucleocapsids, showed that AZ-27 inhibits (IC50 ≈ 15 nM) the synthesis of transcripts from the 3′-end of the genome to a greater extent than those from the 5′-end, indicating that it inhibits transcription initiation. In experiments assaying polymerase activity on the promoter, AZ-27 inhibits transcription and replication initiation. The RSV polymerase also utilizes the promoter sequence to perform a back-priming reaction. Addition of AZ-27 has no effect on the addition of up to three nucleotides by back-priming, but inhibits further extension of the back primed RNA. These findings suggest that the RSV polymerase is likely to adopt different conformations to execute its separate functions at the promoter. |
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| Company Name: |
Energy Chemical
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| Tel: |
021-58432009 400-005-6266 |
| Website: |
http://www.energy-chemical.com |
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