| Identification | Back Directory | [Name]
Phosphine-biotin | [CAS]
608514-42-7 | [Synonyms]
Phosphine-biotin
ABJATKZPSPFIQY-CELQPYBTSA-N
Benzoic acid, 2-(diphenylphosphino)-4-[21-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1,17-dioxo-6,9,12-trioxa-2,16-diazaheneicos-1-yl]-, methyl ester | [Molecular Formula]
C41H53N4O8PS | [MDL Number]
MFCD18428024 | [MOL File]
608514-42-7.mol | [Molecular Weight]
792.92 |
| Hazard Information | Back Directory | [Description]
Phosphine biotin is a labeling reagent that selectively reacts with azido groups on modified proteins through the Staudinger ligation reaction. Modified proteins can be detected using common avidin-based biochemical techniques in whole cells or by blotting experiments following SDS-PAGE. For example, phosphine-biotin has been used successfully in conjunction with DAz-1 or DAz-2 to label and detect sulfenic acid sites in proteins.1,2 | [References]
1. Seo, Y.H., and Carroll, K.S. Facile synthesis and biological evaluation of a cell-permeable probe to detect redox-regulated proteins Bioorg. Med. Chem. Lett. 19(2),356-359(2009).
2. Reddie, K.G., Seo, Y.H., Muse, W.B., III, et al. A chemical approach for detecting sulfenic acid-modified proteins in living cells Mol. Biosyst. 4(6),521-531(2008). |
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