| Identification | Back Directory | [Name]
PROTAMINE SULFATE GRADE III FROM HERRING | [CAS]
9007-31-2 | [Synonyms]
clupeine
protamine sulfate from herring
Protamine sulfate salt herring
protamine sulfate salt from herring
PROTAMINE SULFATE GRADE III FROM HERRING
PROTAMINE SULFATE GRADE III FROM HERRING USP/EP/BP | [EINECS(EC#)]
232-726-0 | [MDL Number]
MFCD00132091 |
| Chemical Properties | Back Directory | [solubility ]
6 M HCl: 25mg/mL, clear, colorless to very faintly yellow | [form ]
powder | [pka]
7.4-8.0; pKb 2.9-3.3(at 25℃) | [biological source]
fish (herring) | [Merck ]
13,2449 |
| Hazard Information | Back Directory | [Uses]
May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer. | [Biochem/physiol Actions]
Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1. | [Purification Methods]
Protamine sulfate is a strongly basic protein (white powder, see pK) used to precipitate nucleic acids from crude protein extracts. It dissolves to the extent of 1.25% in H2O. It is freely soluble in hot H2O but separates as an oil on cooling. It has been purified by chromatography on an IRA-400 ion-exchange resin in the SO 4 form and is washed with dilute H2SO4. Eluates are freeze-dried under high vacuum below 20o. This method is used to convert proteamine and protamine hydrochloride to the sulfate. [UV: Rasmussen Hoppe Seyler's Z Physiol Chem 224 97 1934, Ando & Sawada J Biochem (Tokyo) 49 252 1961, Felix & Hashimoto Hoppe Seyler's Z Physiol Chem 330 205 1963.] |
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