| Identification | Back Directory | [Name]
ALPHA-KETOGLUTARATE DEHYDROGENASE | [CAS]
9031-02-1 | [Synonyms]
α-Ketoglutarate Dehydrogenase
ALPHA-KETOGLUTARATE DEHYDROGENASE
A-ketoglutarate dehydrogenase from*porcine heart
multienzyme 2-oxoglutarate dehydrogenase complex
α-ketoglutarate dehydrogenase from porcine heart
α-Ketoglutarate Dehydrogenase, from porcine heart
alpha-Ketoglutarate Dehydrogenase from porcine heart
MULTIENZYME 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, (1) INCLUDING EC 1.2.4.2 | [MDL Number]
MFCD00130490 |
| Hazard Information | Back Directory | [Uses]
α-Ketoglutarate Dehydrogenase from the porcine heart has been used:
- to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
- to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
- as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons
| [General Description]
α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2). | [Biochem/physiol Actions]
α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease. |
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