| Identification | Back Directory | [Name]
DIHYDROPTERIDINE REDUCTASE FROM SHEEP LI | [CAS]
9074-11-7 | [Synonyms]
DIHYDROPTERIDINE REDUCTASE FROM SHEEP LI
dihydropteridine reductase from*sheep liver | [MDL Number]
MFCD00130957 |
| Hazard Information | Back Directory | [Purification Methods]
Dihydropteridine reductase is purified to homogeneity on a naphthoquinone affinity adsorbent, followed by DEAE-Sephadex and CM-Sephadex chromatography. [Firgaira, Cotton and Danks, Biochem J 197 31 1981.] [For other dihydropteridine reductases see Armarego et al. Med Res Rev 4(3) 267 1984.] 3,4-Dihydroxyphenylalanine-containing proteins. Boronate affinity chromatography is used in the selective binding of proteins containing 3,4-dihydroxyphenylalanine to a m-phenylboronate agarose column and eluted with 1M NH4OAc at pH 10. [Hankus et al. Anal Biochem 150 187 1986.] |
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