100874-08-6
中文名称
SB 4
英文名称
SB 4
CAS
100874-08-6
分子式
C14H10BrNOS
分子量
320.2
MOL 文件
100874-08-6.mol
更新日期
2024/08/06 14:59:37
100874-08-6 结构式
基本信息
中文别名
化合物SB 42-((4-溴苄基)硫代)苯并[D]恶唑
英文别名
SB 4BMP signaling agonist sb4
Benzoxazole, 2-[[(4-bromophenyl)methyl]thio]-
物理化学性质
熔点70-72 °C
沸点444.2±47.0 °C(Predicted)
密度1.57±0.1 g/cm3(Predicted)
储存条件2-8°C
储存条件Sealed in dry,2-8°C
溶解度溶于二甲基亚砜
酸度系数(pKa)0.92±0.10(Predicted)
形态Solid
颜色White to light yellow
常见问题列表
生物活性
SB 4 (Eticovo) 是一种 bone morphogenetic protein 4 (BMP4) signaling 的有效的选择性激动剂，其EC50值为74 nM。SB 4 (Eticovo) 可增强经典的BMP信号传导并激活 SMAD-1/5/9 磷酸化。靶点
| Target | Value |
|
BMP4
(Cell-free assay) | 74 nM(EC50) |
体外研究
BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions. BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4.
Western Blot Analysis
| Cell Line: | Primary mouse kidney epithelial cells (PRECs) |
| Concentration: | 0.05 μM-1 μM |
| Incubation Time: | 24 hours |
| Result: | Incresed p-SMAD-1/5/9 abundance in PRECs. |
Western Blot Analysis
| Cell Line: | BRE-Luc cells |
| Concentration: | 1 μM |
| Incubation Time: | 0min, 5 mins, 15 mins, 30 mins, 45 mins, 60 mins |
| Result: | Acted to stabilize p-SMAD-1/5/9 to enhance the transcriptional response. |