114458-99-0
114458-99-0 结构式
基本信息
中文别名
四甲基罗丹明-4-碘乙酰胺 英文别名
VINNHWDBTRUVKY-UHFFFAOYSA-NTetramethylrhodamine-4-iodoacetamide
Tetramethylrhodamine-5-iodoacetamide dihydroiodide
5-TMRIA [TetraMethylrhodaMine-5-IodoacetaMide Dihydroiodide]
9-[2-Carboxy-4-[(2-iodoacetyl)amino]phenyl]-3,6-bis(dimethylamino)xanthylium inner salt
常见问题列表
生物活性
Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 是一种选择性巯基反应染料，用于通过半胱氨酸残基对蛋白质进行非特异性标记。Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 可用于共价标记 DNA 片段。体外研究
Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios.