117089-08-4
117089-08-4 结构式
物理化学性质
常见问题列表
FW1256 (200 µM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS mRNA and protein in LPS-stimulated RAW264.7 macrophages.
FW1256 (200 µM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS PROTE and protein in LPS-stimulated RAW264.7 macrophages.
FW1256 concentration dependently decreases TNF-α (IC
50
of 61.2 µM), IL-6 (IC
50
of 11.7 µM), PGE2 (IC
50
of 25.5 µM) and NO (IC
50
of 34.6 µM) generation in LPS-stimulated RAW264.7 macrophages and bone marrow-derived macrophages (BMDMs) (IC
50
s of 414.9 µM, 300.2 µM, 4 µM and 9.5 µM for TNF-α, IL-6, PGE2 and NO, respectively) .
FW1256 decreases NF-κB activation as evidenced by reduced cytosolic phospho-IκBα levels and reduces nuclear p65 levels in LPS-stimulated RAW264.7 macrophages treated with FW1256.
RT-PCR
| Cell Line: | RAW264.7 cells |
| Concentration: | 200 µM |
| Incubation Time: | 24.5 hours |
| Result: | Significantly reduced IL-1β, COX-2 and iNOS mRNA in LPS-stimulated RAW264.7 macrophages.. |
Western Blot Analysis
| Cell Line: | RAW264.7 cells |
| Concentration: | 200 µM |
| Incubation Time: | 24.5 hours |
| Result: | Significantly reduced IL-1β, COX-2 and iNOS proteinin LPS-stimulated RAW264.7 macrophages.. |
FW1256 (100 mg/kg; intraperitoneal injection; male C57BL/6 mice) treatment reduces IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice.
| Animal Model: | Male C57BL/6 mice (20-25 g, 6-10 weeks) injected with E. coli lipopolysaccharide (LPS) |
| Dosage: | 100 mg/kg |
| Administration: | Intraperitoneal injection |
| Result: | Reduced IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice. |