135897-06-2
135897-06-2 结构式
基本信息
9,10,11,12-Tetrahydro-9,12-epoxy-1H-diindolo[1,2,3-fg:3',2',1'-kl]pyrrolo[3,4-i][1,6]benzodiazocine-1,3(2H)-dione
9,12-Epoxy-1H-diindolo[1,2,3-fg:3',2',1'-kl]pyrrolo[3,4-i][1,6]benzodiazocine-1,3(2H)-dione, 9,10,11,12-tetrahydro-
物理化学性质
常见问题列表
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Chk1 15 nM (IC 50 ) |
Cdc2 250 nM (IC 50 ) |
PKC 1000 nM (IC 50 ) |
Apoptosis
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SB-218078 (2.5-5 μM; 18 hours; HeLa cells) treatment abrogates G2 cell cycle arrest caused by either γ-irradiation or a topoisomerase I Topotecan inhibition.
SB-218078 (500-625 μM; 96 hours; HeLa and HT-29 cells) treatment significantly increases the cytotoxicity of DNA damage .
Cell Cycle Analysis
| Cell Line: | HeLa cells |
| Concentration: | 2.5 μM, 5 μM |
| Incubation Time: | 18 hours |
| Result: | Abrogated G2 cell cycle arrest caused by γ-irradiation and topoisomerase I inhibition. |
Cell Cytotoxicity Assay
| Cell Line: | HeLa and HT-29 cells |
| Concentration: | 500 nM, 625 nM |
| Incubation Time: | 96 hours |
| Result: | Enhanced cytotoxicity of DNA damage. |
SB-218078 (5 mg/kg; intraperitoneal injection; for 16 hours; C57/Bl6 mice) treatment could promote a strong increase of γ-H2AX and apoptosis throughout the lymphoma, while having no effect on a healthy spleen in Myc-induced lymphomas mouse model.
| Animal Model: | C57/Bl6 mice injected with ARF -/- lymphomas |
| Dosage: | 5 mg/kg |
| Administration: | Intraperitoneal injection; for 16 hours |
| Result: | Promoted a strong increase of γ-H2AX and apoptosis throughout the lymphoma. |