1564249-38-2
中文名称
BJE6-106
英文名称
BJE6-106
(B106)
CAS
1564249-38-2
分子式
C26H23NO2
分子量
381.47
MOL 文件
1564249-38-2.mol
1564249-38-2 结构式
物理化学性质
沸点548.1±50.0 °C(Predicted)
密度1.16±0.1 g/cm3(Predicted)
储存条件-20°C储存
溶解度DMSO:50.0(Max Conc. mg/mL);131.07(Max Conc. mM)
形态Solid
颜色White to off-white
常见问题列表
生物活性
BJE6-106 (B106) 是一种有效的选择性 PKCδ 抑制剂，IC50 值为 0.05 μM，BJE6-106 (B106) 靶向 PKCδ 的选择性是 PKC 同工酶 PKCα 的 1000倍 (IC50=50 μM)。
BJE6-106 (B106) 可以诱导 caspase 依耐性细胞凋亡 (apoptosis)。BJE6-106 (B106) 具有肿瘤特异性作用。靶点
|
PKCδ 0.05 μM (IC 50 ) |
PKCα 50 μM (IC 50 ) |
体外研究
BJE6-106 (B106) (0.2 μM, 0.5 μM; 24-72 hours) suppresses cell survival in melanoma cell lines with NRAS mutations. BJE6-106 (B106) (0.2 μM, 0.5 μM; 6-24 hours) triggers caspase-dependent apoptosis, increases the activity of caspase 3/7, the effect of B106 is greater than rottlerin (10-fold) in SBcl2 cells. BJE6-106 (B106) (0.5 μM; 2-10 hours) activates the MKK4-JNK-H2AX Pathway by inducing MKK4, JNK and H2AX activation at different times in SBcl2 cells.
Cell Viability Assay
| Cell Line: | Melanoma cell lines with NRAS mutations: SBcl2, FM6, SKMEL2, WM1366, WM1361A, and WM852 cells |
| Concentration: | 0.2 μM, 0.5 μM |
| Incubation Time: | 24 hours, 48 hours, or 72 hours |
| Result: | Inhibited cell survival in melanoma cell lines. |
Apoptosis Analysis
| Cell Line: | SBcl2 cells |
| Concentration: | 0.2 μM, 0.5 μM |
| Incubation Time: | 6 hours, 12 hours, or 24 hours |
| Result: | Induced caspase 3/7 activation. |
Western Blot Analysis
| Cell Line: | SBcl2 cells |
| Concentration: | 0.2 μM, 0.5 μM |
| Incubation Time: | 2 hours, 5 hours, 10 hours |
| Result: | Increased phosphorylation of MKK4, JNK and H2AX. |