1694495-59-4
中文名称
Alkyne tyramide
英文名称
Alkyne tyramide
CAS
1694495-59-4
分子式
C13H15NO2
分子量
217.26
MOL 文件
1694495-59-4.mol
更新日期
2023/09/14 14:01:39
1694495-59-4 结构式
基本信息
中文别名
化合物ALKYNE TYRAMIDE 英文别名
Alkyne tyramide4-Pentynamide, N-[2-(4-hydroxyphenyl)ethyl]-
Alkyne tyramide,membrane,mitochondria,topology,Inhibitor,inhibit,APEX2,substrate,yeast
物理化学性质
沸点464.5±35.0 °C(Predicted)
密度1.126±0.06 g/cm3(Predicted)
酸度系数(pKa)10.01±0.15(Predicted)
形态Solid
颜色White to off-white
常见问题列表
生物活性
Alkyne tyramide 是一种抗坏血酸过氧化物酶 2 (APEX2) 探针。Alkyne tyramide 显著提高了完整酵母细胞的 APEX 标记效率，比 APEX2 底物生物素苯酚 (BP) 具有更大的细胞壁渗透性。Alkyne tyramide 还有助于鉴定 APEX 标记位点，明确线粒体蛋白的膜拓扑的分配。体外研究
Yeast cells expressing Su9-APEX2 are incubated with each probe at 2.5 mM final concentration for half an hour, and the labeling reaction is initiated by the addition of 1 mM H2O2. Alkyne tyramide is the most reactive APEX2 substrate for labeling the yeast proteome.
APEX2-mediated Alkyne tyramide labeling in the yeast mitochondria is more effective at probe concentrations above 1 mM.
Alkyne tyramide enables proteomic profiling in the mitochondrial matrix of intact yeast cells with exceptionally high specificity (94%), and offers higher coverage than the traditional APEX2 substrate biotin-phenol (BP).