1875036-74-0
1875036-74-0 结构式
物理化学性质
沸点632.6±55.0 °C(Predicted)
密度1.29±0.1 g/cm3(Predicted)
储存条件-20°C储存
溶解度DMSO : 125 mg/mL (293.08 mM; Need ultrasonic)
酸度系数(pKa)11.59±0.70(Predicted)
形态Solid
颜色White to off-white
常见问题列表
生物活性
BI8622 是一种泛素连接酶 HUWE1 特异性拮抗剂，IC50 为 3.1 μM。靶点
IC50: 3.1 μM (HUWE1)
体外研究
BI8622 induces HUWE1 ectopically expresses to abolish ubiquitination of MCL1 with an IC
50
value of 6.8 μM in HeLa cells.
BI8622 suppresses colony formation of Ls174T cells with estimated IC
50
value of 8.4 μM.
BI8622 (10 μM; 1-4 days) treatment retards passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1.
BI8622 (0-50 μM; 16 hours) retards the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells.
BI8622 inhibits MYC-dependent transactivation in colorectal cancer cells.
Cell Cycle Analysis
| Cell Line: | Ls174T cells |
| Concentration: | 0 μM, 5 μM,10 μM, 15 μM, 20 μM |
| Incubation Time: | 0-4 days |
| Result: | Retarded passage of Ls174T cells through all phases of the cell cycle, with the effect being strongest for G1. |
Western Blot Analysis
| Cell Line: | HeLa cells |
| Concentration: | 0 μM, 10 μM, 20 μM |
| Incubation Time: | 16 hours |
| Result: | Retarded the degradation of MCL1 in response to UV irradiation by inhibiting HUWE1 in HeLa cells. |