188532-26-5

中文名称 NL-1

英文名称 NL 1

CAS 188532-26-5

分子式 C18H25NO3S

分子量 335.46

MOL 文件 188532-26-5.mol

更新日期 2024/07/21 13:45:55

188532-26-5 结构式

基本信息物理化学性质安全数据常见问题列表

基本信息

中文别名

化合物NL-1
5-(3,5-二叔丁基-4-羟基苄基)噻唑烷-2,4-二酮

英文别名

NL 1
2,4-Thiazolidinedione, 5-[[3,5-bis(1,1-dimethylethyl)-4-hydroxyphenyl]methyl]-

物理化学性质

沸点446.5±40.0 °C(Predicted)

密度1.167±0.06 g/cm3(Predicted)

储存条件Sealed in dry,Store in freezer, under -20°C

溶解度DMSO: 250 mg/mL (745.25 mM)

酸度系数(pKa)6.34±0.50(Predicted)

形态Solid

颜色White to off-white

安全数据

危险性符号(GHS)

GHS07

警示词警告

危险性描述H302-H312-H332

防范说明P261-P264-P270-P271-P280-P301+P312-P302+P352-P304+P340-P330-P363-P501

常见问题列表

生物活性

NL-1 是一种具有抗白血病作用的 mitoNEET 抑制剂。NL-1 抑制 REH 和 REH/Ara-C 细胞生长，IC50 分别为 47.35 µM 和 56.26 µM。NL-1 介导的白血病细胞死亡需要激活自噬途径。

靶点

MitoNEET

体外研究

NL-1 (10-100 μM; 72 hours; REH, REH/Ara-C and ALL cell lines) treatment reduces the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner. NL-1 inhibits SUPB15, NALM6 with IC ₅₀ s of 29.48 µM, 94.26 µM, respectively. TOM1, BV173, NALM1 and JM1 all have similar IC ₅₀ values of around 60 µM for NL-1.
NL-1 (60 μM; 6 hours; REH, REH/Ara-C cell lines) treatment mediates autophagy, and inhibition of autophagy partially decreased NL-1-induced tumor cell death.
NL-1 pretreatment inhibits the chemotactic ability of both REH and REH/Ara-C cells to migrate towards multiple chemoattractants. The cells treated with NL1 shows a dose-dependent decrease in chemotaxis both in the REH and the REH/AraC cells.

Cell Proliferation Assay

Cell Line:	REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines
Concentration:	10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, 80 μM, 100 μM
Incubation Time:	72 hours
Result:	Reduced the number of viable cells in REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines, in a concentration-dependent manner.

Cell Autophagy Assay

Cell Line:	REH, REH/Ara-C cell lines
Concentration:	60 μM
Incubation Time:	6 hours
Result:	Induced cell autophagy.

体内研究

NL-1 (10 mg/kg; intraperitoneal injection; daily; for 5 days; female NSG mice) treatment shows antileukemic activity in an in vivo mouse ALL model.

Animal Model:	10 female NOD.Cg- Prkdc ^scid Il2rg ^tm1Wjl /SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells
Dosage:	10 mg/kg
Administration:	Intraperitoneal injection; daily; for 5 days
Result:	Showed antileukemic activity in an in vivo mouse ALL model.