1895050-66-4
1895050-66-4 结构式
物理化学性质
密度1.41±0.1 g/cm3(Predicted)
储存条件-20°C储存
溶解度溶于二甲基亚砜
酸度系数(pKa)8.04±0.70(Predicted)
形态Solid
颜色Off-white to light yellow
常见问题列表
生物活性
6RK73 是一种共价不可逆的特异性 UCHL1 抑制剂，IC50 为 0.23 µM。6RK73 对 UCHL3 几乎没有抑制效果 (IC50=236 µM)。6RK73 特异性抑制乳腺癌中 UCHL1 的活性。靶点
IC50: 0.23 µM (UCHL1), 236 µM (UCHL3)
体外研究
6RK73 (5 µM; 1-3 hours) treatment displays strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels in MDA-MB-436 cells.
6RK73(5 µM; 24-48 hours) results migration significantly slower than the DMSO control group in MDA-MB-436 cells.
Cell Viability Assay
| Cell Line: | MDA-MB-436 cells |
| Concentration: | 5 µM |
| Incubation Time: | 24, 48 hours |
| Result: | Migrated significantly slower than the DMSO control group |
Western Blot Analysis
| Cell Line: | MDA-MB-436 cells |
| Concentration: | 5 µM |
| Incubation Time: | 1, 2, 3 hours |
| Result: | Displayed strong inhibition of the TGFβ-induced pSMAD2 and pSMAD3, and a decrease of TβRI and total SMAD protein levels. |
体内研究
6RK73 displays a potent inhibition of breast cancer extravasation in zebrafish.