205391-02-2
中文名称
205391-02-2
英文名称
4,5-DIAMINOFLUORESCEIN DIACETATE
CAS
205391-02-2
分子式
C24H18N2O7
分子量
446.41
MOL 文件
205391-02-2.mol
205391-02-2 结构式
基本信息
中文别名
化合物 T189664,5-二氨基荧光素二乙酸酯
英文别名
DAF-2 DADAF-2 DIACETATE
DAF-2 DA, CELL PERMEABLE
EZSolution DAF-2 diacetate
5,6-Diaminofluorescein diacetat
4,5-DIAMINOFLUORESCEIN DIACETATE
5,6-DIAMINOFLUORESCEIN DIACETATE
4,5-DIAMINOFLUORORESCEIN DIACETATE
DAF-2 DA - CAS 205391-02-2 - Calbiochem
2-(3,6-DIACETYLOXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID
物理化学性质
沸点719.0±60.0 °C(Predicted)
密度1.54±0.1 g/cm3(Predicted)
闪点87 °C
储存条件2-8°C
溶解度二甲基亚砜：<2mg/毫升
酸度系数(pKa)2.92±0.20(Predicted)
形态淡黄色溶液
颜色白色
常见问题列表
生物活性
DAF-2DA (5,6-Diaminofluorescein diacetat) 是测量 NO 最广泛使用的一种荧光探针。体外研究
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N 2 O 3 , which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).