7733-96-2
![7733-96-2 结构式](/CAS/20210111/GIF/7733-96-2.gif)
基本信息
3,4-Bis(2-thienoyl)-1,2,5-oxadiazole-N-oxide
HC-056456 (3,4-Bis(2-thienoyl)-1,2,5-oxadiazole-N-oxide)
HC-056456 (Synonyms: 3,4-Bis(2-thienoyl)-1,2,5-oxadiazole-N-oxide)
[5-oxido-4-(thiophene-2-carbonyl)-1,2,5-oxadiazol-5-ium-3-yl]-thiophen-2-ylmethanone
物理化学性质
常见问题列表
CatSper
HC-056456 similarly slows the rise of [Ca 2+ ] i that is evoked by alkaline depolarization and reported by fura-2. HC-056456 also selectively and reversibly decreased CatSper currents recorded from patch-clamped sperm. HC-056456 produces a pharmacological phenocopy of the CatSper-null sperm. Acute application of HC-056456 causes rapid loss of flagellar waveform asymmetry from hyperactivated sperm, indicating that continued entry of Ca 2+ through CatSper channels is required to maintain hyperactivation. HC-056456 selectively and reversibly blocks CatSper currents. The specificity and reversibility of the blockade of CatSper-dependent currents by HC-056456 is examined by using patch clamp recordings. The observed current is blocked slightly more than 50% by 20 µM HC-056456 (estimated IC 50 near 15 µM). In concept, it remains possible that CatSper channel heterogeneity explains residual HC-056456-resistant current. The action of HC-056456 on KSper channels, the other major cation channel observed in patch-clamped sperm, is also examined. Subsequent application of 50 µM HC-056456 results in partial blockade of this current. For HC-056456 action on KSper an IC 50 near 40 µM is estimated.