796888-12-5

中文名称 LCH-7749944

英文名称 LCH-7749944

CAS 796888-12-5

分子式 C20H22N4O2

分子量 350.41

MOL 文件 796888-12-5.mol

更新日期 2024/09/03 14:18:03

796888-12-5 结构式

基本信息物理化学性质 LCH-7749944价格(试剂级) 常见问题列表

基本信息

中文别名

化合物LCH-7749944

英文别名

GNF-PF-2356
LCH-7749944
LCH-7749944， GNF-PF-2356
2,4-Quinazolinediamine, N2-(3-methoxyphenyl)-N4-[(tetrahydro-2-furanyl)methyl]-

物理化学性质

沸点580.0±58.0 °C(Predicted)

密度1.272±0.06 g/cm3(Predicted)

储存条件Keep in dark place,Sealed in dry,2-8°C

溶解度DMSO: 250 mg/mL (713.45 mM)

酸度系数(pKa)7.68±0.30(Predicted)

形态Solid

颜色White to off-white

LCH-7749944价格(试剂级)

报价日期	产品编号	产品名称	CAS号	包装	价格
2024/08/19	HY-125035	LCH-7749944 LCH-7749944	796888-12-5	5mg	1980元
2024/08/19	HY-125035	LCH-7749944 LCH-7749944	796888-12-5	10mM * 1mLin DMSO	2178元
2024/08/19	HY-125035	LCH-7749944 LCH-7749944	796888-12-5	10mg	2873元

常见问题列表

生物活性

LCH-7749944 (GNF-PF-2356) 是一种有效的 PAK4 抑制剂，IC50 为 14.93 μM。LCH-7749944通过下调 PAK4/c-Src/EGFR/cyclin D1 途径有效抑制人胃癌细胞的增殖，并诱导凋亡 (apoptosis)。

靶点

PAK4

14.93 μM (IC ₅₀ )

体外研究

LCH-7749944 (GNF-PF-2356; 5-50 μM; 24 hours) inhibits the proliferation of MKN-1, BGC823, SGC7901 and MGC803 cells in a concentration dependent manner.
LCH-7749944 (5-20 μM; 12-48 hours) induces apoptosis of SGC7901 cells.
LCH-7749944 (5-20 μM; 12-48 hours) prominently induces a dose-dependent increase in the percentage of cells in G1 phase and decrease in S phase.
LCH-7749944 (5-30 μM; 24 hours) dramatically decreases levels of phosphoPAK4, phospho-c-Src, phospho-EGFR and cyclin D1 protein expression in a dose-dependent manner.

Cell Proliferation Assay

Cell Line:	MKN-1, BGC823, SGC7901 and MGC803 human gastric cancer cells
Concentration:	5, 10, 15, 20, 25, 30, 35, 40, 45, 50 μM
Incubation Time:	24 hours
Result:	Inhibited the proliferation of MKN-1, BGC823, SGC7901 and MGC803 cells in a concentration dependent manner.

Apoptosis Analysis

Cell Line:	SGC7901 cells
Concentration:	5, 10, 20 μM
Incubation Time:	12, 24, 48 hours
Result:	Induced apoptosis of SGC7901 cells.

Cell Cycle Analysis

Cell Line:	SGC7901 cells
Concentration:	5, 10, 20 μM
Incubation Time:	12, 24, 48 hours
Result:	Prominently induced a dose-dependent increase in the percentage of cells in G1 phase and decrease in S phase.

Western Blot Analysis

Cell Line:	SGC7901 cells
Concentration:	5, 10, 20, 30 μM
Incubation Time:	24 hours
Result:	Dramatically decreased levels of phosphoPAK4, phospho-c-Src, phospho-EGFR and cyclin D1 protein expression in a dose-dependent manner.