83329-73-1
83329-73-1 结构式
常见问题列表
Hygrolidin (0.1, 1 μg/ml; for 24 h) increases both G1 and S phase populations and decreases M phase population.
Hygrolidin (0.1, 1, 10 μg/ml; for 24 h) decreases the amounts of cdk4, cyclin D, cyclin B and increases the amounts of cyclin E and p21. Hygrolidin-induced p21 preferentially associates with cyclin A-cdk2 complex and inhibits it.
Hygrolidin (0.1, 1 μg/ml; for 24 h) selectively induces p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts.
Hygrolidin, for 3 days, inhibits growth of various cell lines including DLD-1 colon cancer, LNCaP prostate cancer, K562 leukemia cells, LNCaP prostate cancer, EL-4 lymphoma (IC
50
s = 1.0-33 ng/ml, respectively).
Hygrolidin shows growth inhibition on
Trypanosoma cruzi
(IC
50
=1.1 nM),
Trypanosoma brucei brucei
(IC
50
=77 nM), and
Leishmania donovani
(IC
50
=72.5 nM).
Cell Cycle Analysis
| Cell Line: | DLD-1 cells |
| Concentration: | 0.1, 1 μg/ml |
| Incubation Time: | For 24 hours |
| Result: | Increased both G1 and S phase populations and decreased M phase population. |
Western Blot Analysis
| Cell Line: | DLD-1 cells |
| Concentration: | 0.1, 1, 10 μg/ml |
| Incubation Time: | For 24 hours |
| Result: | Decreased the amounts of cdk4, cyclin D, cyclin B and increased the amounts of cyclin E and p21. |
RT-PCR
| Cell Line: | DLD-1 or WI-38 cells |
| Concentration: | 0.1, 1 μg/ml |
| Incubation Time: | For 24 hours |
| Result: | Selectively induced p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts. |