85138-49-4
![85138-49-4 结构式](/CAS/GIF/85138-49-4.gif)
基本信息
2,7-双-(羧乙基)-羧基-荧光素
2,7-双(2-羧乙基)-5(6)-羧基荧光素
2`,7`-双(2-羧基乙基)-5(6)-羧基荧光素
2',7'-二-(2-羧乙基)-5(6)-羧基荧光素
5(OR 6)-羧基-3',6'-二羟基-3-氧代-螺[异苯并呋喃-1(3H),9'-[9H]氧杂蒽]-2',7'-二丙酸
5(OR6)-羧基-3',6'-二羟基-3-氧代-螺[异苯并呋喃-1CHEMICALBOOK(3H),9'-[9H]氧杂蒽]-2',7'-二丙酸
BCECF acid *CAS#: 85138-49-4*
2',7'-Biscarboxyethyl-6-carboxyfluorescein
2',7'-BIS-(CARBOXYETHYL)-CARBOXY-FLUORESCEIN
2,7-Bis(2-carboxyethyl)-6-carboxyfluoresceine
2',7'-Bis(carboxyethyl)-4or5-carboxyfluorescein
2'7'-BIS-(2-CARBOXYETHYL)-5-(AND-6) FLUORESCEIN
2',7'-BIS(CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN
2',7'-BIS-(CARBOXYETHYL)-5(6')-CARBOXYFLUORESCEIN
2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN
物理化学性质
报价日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
2024/01/25 | HY-101882 | 2,7-双(2-羧乙基)-5(6)-羧基荧光素 BCECF | 85138-49-4 | 1mg | 1600元 |
常见问题列表
Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K + /H + ionophor nigericin does not inhibit hydrogenosomal loading with BCECF.The pH-sensitive fluorescent dyes to measure cytosolic pH. 1.Prepare a 2 to 20 mM stock solution of BCECF in DMSO. 2.Prepare a 5-50 µM BCECF dye-loading solution in buffer solutions (HHBS or PBS).3. Add 1000 µL/well (6-well plate),100 µL/well (96-well plate) or 25 µL/well (384-well plate) BCECF dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator for 30-60 minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at E x /E m = 490/535 nm or 430/535 nm for ratio measurements.