ANTI-EGFR抗体性质、用途与生产工艺
兔单克隆抗体 to EGFR
Rabbit
与反应: Mouse, Rat, Human
Unconjugated
Produced in rabbits immunized with purified, recombinant Mouse EGFR / HER1 / ErbB1 (rM EGFR / HER1 / ErbB1; Catalog#51091-M08H; Q01279; Met1-Ser647). EGFR / HER1 / ErbB1 specific IgG was purified by Mouse EGFR / HER1 / ErbB1 affinity chromatography.
适用于: WB, IP, Flow Cyt, IHC-P, ICC/IF
Western blot - Anti-EGFR antibody [EP38Y] (ab52894)
All lanes : Anti-EGFR antibody [EP38Y] (ab52894) at 1/10000 dilution (purified)
Lane 1 : Rat liver lysates
Lane 2 : Mouse lung lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution Predicted band size: 134 kDa Observed band size: 175 kDa
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
双抗体夹心法测定标本中EGFR水平。用纯化的EGFR抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入EGFR，再与HRP标记的EGFR抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的EGFR呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中EGFR浓度。
Anti-EGFR Antibody是一类可以特异性结合EGFR的多克隆抗体，主要用于检测EGFR的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。
ANTI-EGFR抗体
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