Anti-FITC抗体

Anti-FITC抗体性质、用途与生产工艺

描述

兔多克隆抗体to FITC荧光(HRP)

宿主

Rabbit

种属反应性

Species independent

偶联性

HRP

背景

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

应用

IHC-P, WB

产品简介

FITC（异硫氰酸荧光素）标记抗体是将FITC与特异性抗体经适当方法连接而成，FITC（异硫氰酸荧光素）能与各种抗体蛋白结合，结合后的抗体不丧失与一定抗原结合的特异性，其应用范围非常广泛，结果即时可见、敏感性高。

下游应用

Anti-FITC抗体是一类可以特异性结合FITC的单克隆抗体，主要用于检测FITC的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。

检测原理

双抗体夹心法测定标本中FITC水平。用纯化的FITC抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入FITC，再与HRP标记的FITC抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的FITC呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中FITC浓度。

Anti-FITC抗体 上下游产品信息

上游原料

下游产品

Anti-FITC抗体 生产厂家

 

Anti-FITC抗体 相关搜索:

• 兔抗荧光素抗体
• ANTI-FITC抗体
• 异硫氰酸荧光素标记蛋白激酶B抗体(标记抗体)
• Anti-FITC Antibody