小鼠抗EGFR单克隆抗体
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- CAS号:
- 英文名:
- Anti-EGFR mouse monoclonal antibody
- 英文别名:
- Anti-EGFR mouse monoclonal antibody;Mouse anti-EGFR Monoclonal Antibody
- 中文名:
- 小鼠抗EGFR单克隆抗体
- 中文别名:
- 小鼠抗EGFR单克隆抗体
- CBNumber:
- CB54711826
- 分子式:
- 分子量:
- 0
- MOL File:
- Mol file
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小鼠抗EGFR单克隆抗体性质、用途与生产工艺
Anti-EGFR Antibody, Mouse Monoclonal
Mouse
Human
IHC-Fr, ICC/IF, Flow Cyt
Recombinant Human EGFR / HER1 / ErbB1 protein
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Immunocytochemistry/ Immunofluorescence - Anti-EGFR antibody [EGFR1] (ab30)
ab30 staining EGFR (colored green) in A431 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab30 at 5μg/ml overnight at 4ºC. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
EGFR是表皮生长因子受体(HER)家族成员之一。该家族包括HER1(erbB1,EGFR)、HER2(erbB2,NEU)、HER3(erbB3)及HER4(erbB4)。HER家族在细胞生理过程中发挥重要的调节作用。
EGFR(Epidermal Growth Factor Receptor)是上皮生长因子(EGF)细胞增殖和信号传导的受体。EGFR属于ErbB受体家族的一种,该家族包括EGFR (ErbB-1),HER2/c-neu(ErbB-2),Her 3(ErbB-3) 和Her 4(ErbB-4)。EGFR也被称作HER1、ErbB1,突变或过表达一般会引发肿瘤。EGFR是一种糖蛋白,属于酪氨酸激酶型受体,细胞膜贯通,分子量170KDa。EGFR位于细胞膜表面,靠与配体结合来激活,包括EGF和TGFα(transforming growth factor α)。激活后,EGFR由单体转化为二聚体,尽管也有证据表明,激活前也存在二聚体。
小鼠抗EGFR单克隆抗体是一类可以特异性结合EGFR的单克隆抗体,主要用于检测EGFR的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。
双抗体夹心法测定标本中EGFR水平。用纯化的Anti-ACTA2抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入EGFR,再与HRP标记的EGFR抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的EGFR呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中EGFR浓度。
小鼠抗EGFR单克隆抗体
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- 小鼠抗EGFR单克隆抗体
- Anti-EGFR mouse monoclonal antibody
- Mouse anti-EGFR Monoclonal Antibody