PERCP标记小鼠抗人CD3单克隆抗体性质、用途与生产工艺
Anti-CD3 epsilon/CD3e Antibody (PerCP), Mouse Monoclonal
Mouse
Human
Recombinant Human CD3e / CD3 epsilon protein
FCM
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3e / CD3 epsilon and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.
Anti-CD3 epsilon/CD3e Antibody (PerCP), Mouse Monoclonal---Sino Biological
Flow cytometric analysis of Human CD3 expression on human peripheral blood lymphocytes. Cells were stained with PerCP-conjugated anti-Human CD3 and APC conjugated anti-Human CD19 (BD Pharmingen™ Cat. No. 555415). The dot plots were derived from events with the forward and side light-scatter characteristics of viable lymphocytes.
T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
在免疫学中，CD3（分化簇3）T细胞的共受体是一种蛋白质复合物，它由四个不同的链组成。在哺乳动物中，该复合物含有一个CD3γ链，CD3δ链，和2 CD3ε链。这些链具有被称为一个分子副T细胞受体（TCR）和ζ-链以产生激活信号的T淋巴细胞。该TCR，ζ链和CD3分子一起构成的T细胞受体复合物。
CD3分子是T细胞的重要标志，由γ、δ、ε、ζ和η五种多肽链组成，它们的胞浆区均含有ITAM，具有传导TCR信号的功能，使T细胞活化。CD3分子与TCR以非共价键结合形成TCR-CD3复合物，其主要功能是把TCR与抗原结合后产生的活化信号传递到细胞内，诱导T细胞活化。
PERCP标记小鼠抗人CD3单克隆抗体是一类可以特异性结合CD3的单克隆抗体，主要用于检测CD3的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。
双抗体夹心法测定标本中CD3水平。用纯化的CD3抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CD3，再与HRP标记的CD3抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CD3呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中CD3浓度。
PERCP标记小鼠抗人CD3单克隆抗体
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