ANTI-BDNF抗体

ANTI-BDNF抗体性质、用途与生产工艺

描述

兔单克隆抗体to BDNF

宿主

Rabbit

种属反应性

Mouse, Rat, Human

免疫原

Synthetic peptide within Human BDNF aa 150 to the C-terminus. The exact sequence is proprietary.

验证的应用

Flow Cyt, WB, IHC-P, IHC-Fr, ICC/IF

制备方法

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse BDNF/ANON2.

品牌示例

Immunohistochemistry (Frozen sections) - Anti-BDNF antibody(ab108319)

IHC image of BDNF staining in a section of frozen normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab108319, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

背景

BDNF is a member of the nerve growth factor family. It is highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. It also can be detected in heart, lung, skeletal muscle, testis, prostate and placenta. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. During development, BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. It participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. It functions as the major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

简介

ANTI-BDNF抗体是以ANTI-BDNF为抗原以兔为宿主制得的免疫抗体，可以特异性结合BDNF。主要用于检测BDNF的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。

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ANTI-BDNF抗体 相关搜索:

• ANTI-BDNF抗体
• Anti-BDNF antibody