ANTI-SF1 抗体

ANTI-SF1 抗体性质、用途与生产工艺

描述

兔单克隆抗体[EPR9105(B)] to Tat-SF1

宿主

Rabbit

种属反应性

与反应: Rat, Human

制备

Produced in rabbits immunized with E. coli-derived Human HTATSF1 fragment, and purified by antigen affinity chromatography.

验证的应用

WB,IHC-P,ICC/IF,IP

品牌示例

All lanes : Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 86 kDa



Western blot - Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921)

背景

This gene encodes a nuclear pre-mRNA splicing factor. The encoded proteinspecifically recognizes the intron branch point sequence at the 3' splice site,together with the large subunit of U2 auxiliary factor (U2AF), and is required for theearly stages of spliceosome assembly. It also plays a role in nuclear pre-mRNAretention and transcriptional repression. The encoded protein contains an N-terminalU2AF ligand motif, a central hnRNP K homology motif and quaking 2 region whichbind a key branch-site adenosine within the branch point sequence, a zinc knucklesdomain, and a C-terminal proline-rich domain. Alternative splicing results in multipletranscript variants.

检测原理

双抗体夹心法测定标本中SF1水平。用纯化的SF1抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入SF1，再与HRP标记的SF1抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的SF1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中SF1浓度。

解旋酶

解旋酶是最大的一类酶，几乎所有涉及DNA或RNA的生物过程都使用一种或多种解旋酶。很多疾病与解旋酶的功能缺陷以及蛋白质表达失控有关，包括癌症、发育缺陷和神经退行性疾病。分为环状为主的六面体结构，由SF3至6组成，未形成环的酶由SF1和2组成。以前基于序列、结构和机制特征分类，但这些分类都没有扩展到SF1和SF2的所有成员，本文基于不同的蛋白质家族分类。
超家族SF1和2的解旋酶几乎参与RNA和DNA代谢的各个方面，有相似的催化核心结构。基于蛋白质家族的分类，具有典型的序列、结构和机制特征。这个分类补充和扩展了现有的SF1和SF2解旋酶分类，并突出蛋白质的主要结构和功能。SF1和SF2解旋酶的标志是一个保守的解旋酶核心，由两个相似的蛋白结构域组成，类似于重组蛋白RecA的折叠，含有特征序列基序，在此基础上完成了解旋酶在SFS中的原始细分。

ANTI-SF1 抗体 上下游产品信息

上游原料

下游产品

ANTI-SF1 抗体 生产厂家

 

ANTI-SF1 抗体 相关搜索:

• 抗体-一抗
• ANTI-SF1ANTIBODY -剪接因子1重组兔单克隆抗体
• ANTI-SF1 抗体
• Anti-Uqcr5
• Anti-Ubiquinol-cytochrome c reductase, rieske iron-sulfur polypeptide 1
• Anti-Risp
• Anti-UQCRFS1, (N-terminal) antibody produced in rabbit
• sf-1
• sf1
• Nuclear receptor elp
• ftzf1
• Anti-NR5A1 antibody produced in rabbit
• Ad4bp
• Anti-NR5A1 (AB3) antibody produced in rabbit
• Anti-NR5A1 (111-125) antibody produced in rabbit
• Anti-Nuclear receptor subfamily 5, group A, member 2
• Anti-NR5A2 (AB1) antibody produced in rabbit
• Anti-FTZ-F1beta
• Anti-CPF
• Anti-B1F2
• Anti-B1F
• Anti-Nuclear receptor subfamily 5, group A, member 1
• Anti-NR5A1 (431-442) antibody produced in rabbit
• Anti-FTZF1
• Anti-FTZ1
• Anti-ELP
• Anti-AD4BP
• Anti-Zinc finger protein 162
• Anti-Zinc finger gene in MEN1 locus
• Anti-Transcription factor ZFM1
• Anti-Mammalian branch point-binding protein mBBP
• Anti-BBP
• Anti-Tumor suppressor p53-binding protein 2
• Anti-TP53BP2 antibody produced in rabbit
• Anti-Renal carcinoma antigen NY-REN-51
• Anti-p53BP2
• Anti-p53-binding protein 2
• Anti-Bcl2-binding protein
• Anti-Apoptosis-stimulating of p53 protein 2
• Anti-NR5A1 (AB1) antibody produced in rabbit
• Anti-NR5A2 (AB2) antibody produced in rabbit
• Anti-TM2D1 antibody produced in rabbit
• Anti-TM2 domain containing 1
• Anti-NR5A2, (C-terminal) antibody produced in rabbit
• Anti-TMEM158 antibody produced in rabbit
• Anti-RIS1
• Anti-p40BBP
• Anti-DKFZp586E1621
• Anti-LRH-1
• Anti-Ftf
• Anti-D1Ertd308e
• Anti-AU020803
• Anti-SF1 (AB1) antibody produced in rabbit
• Anti-SF1 antibody produced in rabbit
• ANTI-SF1
• ANTI-SPLICING FACTOR 1