TRANSCRIPTOR REVERSE TRANSCRIPTASE

Uses

Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler^? Instruments) for the following applications:

• Synthesis of first-strand cDNA for use in subsequent amplification reactions
• RT-PCR of GC-rich RNA templates
• Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
• Retrieving and cloning the 5′ and 3′ termini of mRNA by RACE
• Generation of cDNA libraries with large inserts
• Dideoxy DNA sequencing
• RNA sequencing
• 3′-end labeling of DNA fragments
• Generation of single-stranded probes for genomic footprints
• In the reverse transcription of RNA from human Papillomavirus E6, cortical and striatal tissues, muscle biopsies of Becker muscular dystrophy samples and miRNA stem-loop from oocytes and human cerebral microvascular endothelial cells (hCMVECs)

General Description

In retroviruses like the human immunodeficiency virus type 1 (HIV-1), reverse transcriptase (RT) is the core enzyme. HIV-1 RT is made of two subunits of 66?kDa and 51?kDa (p66 and p5l). A human endogenous retrovirus of the HERV-K family codes for reverse transcriptase (RT) enzyme.

Biochem/physiol Actions

Human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) is essential for the catalytic conversion of single-stranded viral RNA into the double-stranded linear DNA that is integrated into host cell chromosomes.