Methyl Green
- CAS No.
- 82-94-0
- Chemical Name:
- Methyl Green
- Synonyms
- CI 42585;LIGHT GREEN;PARIS GREEN;CI NO 42585;CI NO 42590;METHYL GREEN;DOUBLE GREEN;BASIC BLUE 20;DOUBLE GREEN SF;CI BASIC BLUE 20
- CBNumber:
- CB0389594
- Molecular Formula:
- C26H33ClN3+
- Molecular Weight:
- 423.02
- MDL Number:
- MFCD00011879
- MOL File:
- 82-94-0.mol
| Water Solubility | Soluble in water |
|---|---|
| solubility | Methanol (Slightly), Water (Slightly) |
| Colour Index | 42585 |
| form | Liquid |
| color | Clear blue-green |
| PH Range | 0.1(yellow)-2.3(blue) |
| Stability | Hygroscopic |
| CAS DataBase Reference | 82-94-0 |
| EWG's Food Scores | 1 |
| FDA UNII | 6P7U4T9BXA |
| EPA Substance Registry System | C.I. Basic Blue 20 (82-94-0) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H335-H315-H319-H413 |
| Precautionary statements | P264-P280-P305+P351+P338-P337+P313P-P264-P280-P302+P352-P321-P332+P313-P362 |
| HS Code | 3204.13.8000 |
Methyl Green price More Price(7)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 198080 | Methyl Green zinc chloride salt, certified by the Biological Stain Commission, Dye content 85?% | 82-94-0 | 10g | $130 | 2021-12-16 | Buy |
| Sigma-Aldrich | 198080 | Methyl Green zinc chloride salt, certified by the Biological Stain Commission, Dye content 85?% | 82-94-0 | 50g | $660 | 2021-12-16 | Buy |
| TCI Chemical | M0498 | Methyl Green | 82-94-0 | 25g | $214 | 2024-03-01 | Buy |
| TRC | B400378 | [4-[[4-(Dimethylamino)phenyl]-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethylazaniumDichloride | 82-94-0 | 250mg | $45 | 2021-12-16 | Buy |
| Chem-Impex | 38658 | Methylgreen,CI42585 | 82-94-0 | 25G | $234.12 | 2021-12-16 | Buy |
Methyl Green Chemical Properties,Uses,Production
Description
Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently.
Uses
Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronine Y dye for one stage DNA (green) and RNA (red) staining method. It is also used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann. Methyl green solution is a suitable counterstain for chloroacetate esterase, nonspecific esterase, alkaline phosphatase, peroxidase, naphthol AS acetate esterase, and acid phosphatase.
Preparation
chlorine methane and (C.I.Basic Violet 1, C.I. 42535) reaction
Definition
ChEBI: Methylene green is an organic chloride salt having 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium as the counterion. It stains nuclei green and is sometimes used as a counterstain to red or purple primary stains. It has a role as a fluorochrome and a histological dye. It contains a 3,7-bis(dimethylamino)-4-nitrophenothiazin-5-ium.
Properties and Applications
green light blue. Soluble in water for blue light green, not soluble in ethanol and e alcohol. The strong sulfuric acid to red light yellow, yellow green for diluted. Dyes to join sodium hydroxide solution to colorless.
| Standard( Cotton ) | Light Fastness | Persperation Fastness | Ironing Fastness | Soaping | |||
| Fading | Stain | Fading | Stain | Fading | Stain | ||
| C | 1 | 2 | |||||
Purification Methods
Crystallise the dye from hot water. [Beilstein 13 IV 2286.]
Methyl Green Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Hubei Jusheng Technology Co.,Ltd. | 18871490254 | linda@hubeijusheng.com | CHINA | 28172 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49374 | 58 |
| Career Henan Chemica Co | +86-0371-86658258 +8613203830695 | laboratory@coreychem.com | China | 30236 | 58 |
| Alfa Chemistry | Info@alfa-chemistry.com | United States | 24072 | 58 | |
| SUZHOU SENFEIDA CHEMICAL CO.,LTD | +86-0512-83500002 +8615195660023 | sales@senfeida.com | China | 23046 | 58 |
| Shanghai Acmec Biochemical Technology Co., Ltd. | +86-18621343501; +undefined18621343501 | product@acmec-e.com | China | 33338 | 58 |
| Aladdin Scientific | +1-+1(833)-552-7181 | sales@aladdinsci.com | United States | 57505 | 58 |
| SHANGHAI KEAN TECHNOLOGY CO., LTD. | +8613817748580 | cooperation@kean-chem.com | China | 40066 | 58 |
| J & K SCIENTIFIC LTD. | 010-82848833 400-666-7788 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 021-61259108 18621169109 | market03@meryer.com | China | 40228 | 62 |
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