Uses
Anti-PP2A B subunit isoform B56-gamma Antibody is an antibody against PP2A B subunit isoform B56-gamma for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.', 'Research Category
Signaling', 'Research Sub Category
Kinases & Phosphatases', 'Western Blotting Analysis: A representative lot detected the expression of exogenously transfected human PP2A B56γ subunit in HeLa cells, but not in cells co-transfected with B56γ shRNA (Lee et al., 2010; Zimmerman et al, 2012). Western Blotting Analysis: A representative lot detected exogenously expressed human Bμα1 (PPP2R5C spliced isoform Gamma-3) protein in SF9 insect cells and COS-1 cells, as well as the endogenous r at Bμα1 following its isolation by immunopreciptation from a r at fibroblast cell line, clone 6 (Okamoto, K., et al. (1996). Mol. Cell. Biol. 16(11):6593-6602).Immunocytochemistry Analysis: A representative lot detected differential PP2A B56γ3 subunit cytoplamic and nuclear distribution patterns by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HeLa cells at different stages of cell cycle (Lee, T.Y., et al. (2010). J. Biol. Chem. 285:21567-21580).Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed human Bμα1 (PPP2R5C spliced isoform Gamma-3) from SF9 insect cells and COS-1 cells, as well as the endogenous r at Bμα1 from a r at fibroblast cell line, clone 6 (Okamoto, K., et al. (1996). Mol. Cell. Biol. 16(11):6593-6602).
