Uses
Anti-poly-ADP-ribose binding reagent is a reagent th at selectively binds to both mono- and poly- ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot.', 'Dot Blot Specificity Analysis: This reagent detected mono(ADPR) on recombinant PARP3 protein, as well as mono(ADPR) and poly(ADPR) on recombinant PARP1 recombinant protein (Lee Kraus, University of Texas Southwestern Medical Center).Immunoprecipitation Analysis: A representative lot of Anti-pan-ADP-ribose binding reagent immunoprecipitated ADP-ribosylated proteins from nuclear extract (Lee Kraus, University of Texas Southwestern).Western Blotting Analysis: A representative lot detected auto-ADP-ribosylation activity of PARP1/2/3 and mutants in the presence of NAD+ or various NAD+ analogs (Gibson, B.A., et al. (2016). Science. 353(6294):45-50).Western Blotting Analysis: A representative lot detected PARP1-catalyzed NELF-E ADP-ribosylation in cell-free enzymatic reactions as well as ADP-ribosylation of exogenously expressed FLAG-tagged NELF-E in HEK293T cells. PARP inhibitor PJ34 or P-TEFb/CDK9 inhibitor flavopiridol treatment decreased cellular NELF-E ADP-ribosylation level (Gibson, B.A., et al. (2016). Science. 353(6294):45-50).', 'Research Category
Epigenetics & Nuclear Function', 'Research Sub Category
General Post-translation Modification
