Collagenase
- CAS No.
- 9001-12-1
- Chemical Name:
- Collagenase
- Synonyms
- FIBROBLAST;Collagenase IV;Collagenase clostridium histolyticum;Santyl;INTERSTITIAL;Collagenase A;Collagenase II;COLLAGENASE TYPE XI;COLLAGENASE TYPE VII HIGH PURITY;CLGN
- CBNumber:
- CB2112176
- Molecular Formula:
- C60H100N2
- Molecular Weight:
- 849.4494
- MDL Number:
- MFCD00130830
- MOL File:
- 9001-12-1.mol
| storage temp. | -20°C |
|---|---|
| solubility | Soluble in aqueous buffers. |
| form | powder |
| color | light brown |
| PH | 7.0 |
| Merck | 2481 |
| InChIKey | YRQNKMKHABXEJZ-UVQQGXFZSA-N |
| CAS DataBase Reference | 9001-12-1 |
| EWG's Food Scores | 1 |
| NCI Dictionary of Cancer Terms | collagenase; fibroblast |
| FDA UNII | 9X7O8V25IT |
| NCI Drug Dictionary | collagenase |
| ATC code | D03BA02,D03BA52,M09AB02 |
| EPA Substance Registry System | Collagenase (9001-12-1) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS07,GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H315-H319-H334-H335 |
| Precautionary statements | P261-P264-P280-P284-P304+P340+P312-P342+P311 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| WGK Germany | 1 |
| F | 10-21 |
| HS Code | 3507907000 |
Collagenase price More Price(99)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | COLLA-RO | Collagenase A from Clostridium histolyticum | 9001-12-1 | 100mg | $86.8 | 2024-03-01 | Buy |
| Sigma-Aldrich | COLLA-RO | Collagenase A from Clostridium histolyticum | 9001-12-1 | 500mg | $324 | 2024-03-01 | Buy |
| Sigma-Aldrich | COLLA-RO | Collagenase A from Clostridium histolyticum | 9001-12-1 | 2.5g | $1020 | 2024-03-01 | Buy |
| Sigma-Aldrich | C8176 | Collagenase from Clostridium histolyticum Sigma Blend Type L, ≤1.0?FALGPA units/mg solid | 9001-12-1 | 5G | $5710 | 2024-03-01 | Buy |
| Sigma-Aldrich | C2799 | Collagenase from Clostridium histolyticum powder, suitable for cell culture, ≥4?FALGPA units/mg solid, high purity, ≥700?CDU/mg solid (CDU = collagen digestion units) | 9001-12-1 | 7500units | $228 | 2024-03-01 | Buy |
Collagenase Chemical Properties,Uses,Production
Description
Collagenases are a category of enzymes that can digest native collagen, the major fibrous component of animal extracellular connective tissue. Collagenases can be produced by some bacterias such as Clostridium and can also be made by the animal body as part of its normal immune response. They normally target the connective tissue in muscle cells and other body organs. They can break peptide bonds within collagen and help create collagen by cleaving pro-collagen, collagen's precursor, once it is secreted by the cell. Bacterial collagenases exhibit broader substrate specificity than animal collagenases and unlike animal collagenases that split collagen in its native triple-helical conformation, bacterial collagenase can degrade both water-insoluble native collagens and water-soluble denatured ones. In addition, bacterial collagenase can attack almost all collagen types, and is able to make multiple cleavages within triple helical regions. Collagenases have a molecular weight ranging from 68 kilodaltons (kDa) to 130 Kda due to differing sizes based on the class to which the isolate belongs. The optimal pH for stability ranges from approximately 6.3 to 7.5 with a theoretical isoelectric point of 5.62. Numerous compounds and molecules have the capability of acting as collagenases activator or inhibitor. For example, Ca2+ and Zn2+ ions are two known activators. On the other hand, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), cysteine, histidine, dithiothreitol (DTT), 2-mercaptoethanol, o-phenanthroline, Hg2+, Pb2+, Cd2+, Cu2+, and Zn2+ are all known inhibitors of collagenase activity.
References
1.https://en.wikipedia.org/wiki/Collagenase
2.http://www.wisegeek.com/what-is-collagenase.htm
3.http://www.rockland-inc.com/collagenase.aspx
4.http://www.worthington-biochem.com/cls/default.html
Uses
This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.
Uses
In investigation of the structure and biosynthesis of collagen; in dispersion of cells for tissue culture studies.
Uses
Collagenase, Type I, Clostridium histolyticum is used as a powder and solvent injection kit for the treatment of dupuytren's contracture.
Definition
A rare proteinase that catalyzes the hydrolysis of collagen. Collagenase activity has been demonstrated in several venoms of crotalid and viperid snakes and it occurs in certain species of clostridium.
General Description
Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.
Biochem/physiol Actions
Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.
Purification Methods
Collagenase is purified by using N-ethylmaleimide to activate the enzyme, and wheat germ agglutinin-agarose affinity chromatography [Callaway et al. Biochemistry 25 4757 1986].
Collagenase Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Aladdin Scientific | +1-+1(833)-552-7181 | sales@aladdinsci.com | United States | 52925 | 58 |
| Hebei Weibang Biotechnology Co., Ltd | +8615531157085 | abby@weibangbio.com | China | 8810 | 58 |
| Hebei Mojin Biotechnology Co., Ltd | +86 13288715578 +8613288715578 | sales@hbmojin.com | China | 12839 | 58 |
| Hebei Yanxi Chemical Co., Ltd. | +8617531190177 | peter@yan-xi.com | China | 5856 | 58 |
| Hebei Weibang Biotechnology Co., Ltd | +8617732866630 | bess@weibangbio.com | China | 18153 | 58 |
| Shaanxi TNJONE Pharmaceutical Co., Ltd | +8618092446649 | sarah@tnjone.com | China | 1143 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21634 | 55 |
| Hubei Jusheng Technology Co.,Ltd. | 18871490254 | linda@hubeijusheng.com | CHINA | 28172 | 58 |
| Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 | sales@chemdad.com | China | 39894 | 58 |
| Zhengzhou Alfa Chemical Co.,Ltd | +8618530059196 | sale04@alfachem.cn | China | 13513 | 58 |
View Lastest Price from Collagenase manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2024-11-22 | Collagenase
9001-12-1
|
US $0.00-0.00 / g | 1g | 98.0% | 1kg/month | WUHAN FORTUNA CHEMICAL CO., LTD | |
 |
2024-11-20 | Collagenase
9001-12-1
|
US $19.00 / ASSAYS | 100ASSAYS | 99% | 100 mt | Hebei Weibang Biotechnology Co., Ltd | |
 |
2024-11-19 | Collagenase Type I
9001-12-1
|
US $55.00-39.00 / mg | 10g | TargetMol Chemicals Inc. |
-
- Collagenase
9001-12-1
- US $0.00-0.00 / g
- 98.0%
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- Collagenase
9001-12-1
- US $19.00 / ASSAYS
- 99%
- Hebei Weibang Biotechnology Co., Ltd
-
- Collagenase Type I
9001-12-1
- US $55.00-39.00 / mg
- TargetMol Chemicals Inc.