Ribonuclease H (RNase H)
- CAS No.
- Chemical Name:
- Ribonuclease H (RNase H)
- Synonyms
- Ribonuclease H (RNase H)
- CBNumber:
- CB24670579
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
Ribonuclease H (RNase H) price More Price(1)
Ribonuclease H (RNase H) Chemical Properties,Uses,Production
Uses
Ribonuclease H (RNase H) has been used for:
- In vivo RNA-primed initiation of DNA synthesis
- Elimination of mRNA during second-strand cDNA synthesis
- Site-specific cleavage of RNA
- Detection of RNA:DNA regions in double-stranded DNA of natural origin
- Removal of poly (A) sequences of mRNA if oligo (dT) is present
- RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)
General Description
Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.
Source: E. coli H560 pol A1
Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.
Biochem/physiol Actions
Ribonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Using RNase H for degrading mRNA results in 80% depletion of mRNA and protein expression. RNase H recognizes the start codon and the 3′ and 5′ untranslated regions. This enzyme participates in DNA replication.
Ribonuclease H (RNase H) Preparation Products And Raw materials
Raw materials
Preparation Products
Ribonuclease H (RNase H) Suppliers
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Sigma-Aldrich | 021-61415566 800-8193336 | orderCN@merckgroup.com | China | 51471 | 80 |
Supplier | Advantage |
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Sigma-Aldrich | 80 |