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TRIS hydrochloride

TRIS hydrochloride structure

CAS No.: 1185-53-1

Chemical Name:: TRIS hydrochloride

Synonyms: TRIS-HCL;Tris-HCI;TRIZMA HYDROCHLORIDE;TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE;TRIS-HCL BUFFER;TROMETHAMINE HYDROCHLORIDE;Tris hydrochloric acid;TRIZMA HYDROCHLORIDE REAGENT GRADE;TRI(HYDROXYMETHYL) AMINO METHANE HYDROCHLORIDE;2-amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride

CBNumber:: CB2677770

Molecular Formula:: C4H12ClNO3

Molecular Weight:: 157.6

MDL Number:: MFCD00012590

MOL File:: 1185-53-1.mol

MSDS File:: SDS

Last updated:2024-11-19 20:33:22

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TRIS hydrochloride Properties

Melting point	150-152 °C
Boiling point	225℃[at 101 325 Pa]
Density	1.05 g/mL at 20 °C
vapor pressure	0Pa at 20℃
storage temp.	room temp
solubility	H₂O: 4 M at 20 °C, clear, colorless
form	crystalline
color	clear colorless (40 % (w/w) in H₂O) solution
PH Range	7.0 - 9.0
PH	2.5-4.0 (25℃, 4M in H2O)
pka	8.1(at 25℃)
Water Solubility	SOLUBLE
Sensitive	Hygroscopic
λmax	λ: 260 nm Amax: 0.12 λ: 280 nm Amax: 0.08
BRN	3675235
Stability	Stable. Incompatible with strong oxidizing agents, bases, moisture.
InChIKey	QKNYBSVHEMOAJP-UHFFFAOYSA-N
LogP	-3.6 at 20℃
CAS DataBase Reference	1185-53-1(CAS DataBase Reference)
FDA UNII	383V75M34E
EPA Substance Registry System	1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride (1185-53-1)

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS07

Signal word

Warning

Hazard statements

H315-H319-H335

Precautionary statements

P305+P351+P338

Hazard Codes

Risk Statements

36/37/38

Safety Statements

26-36

WGK Germany

RTECS

TY2900000

TSCA

Yes

HS Code

29221980

NFPA 704

	1
2		1

TRIS hydrochloride price More Price(133)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	T3253	Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline	1185-53-1	50KG	$7730	2024-03-01	Buy
Sigma-Aldrich	T15760	Trizma® hydrochloride reagent grade, ≥99%, crystalline	1185-53-1	500G	$113	2024-03-01	Buy
Sigma-Aldrich	T15760	Trizma® hydrochloride reagent grade, ≥99%, crystalline	1185-53-1	1KG	$210	2024-03-01	Buy
Sigma-Aldrich	T15760	Trizma® hydrochloride reagent grade, ≥99%, crystalline	1185-53-1	5KG	$895	2024-03-01	Buy
Sigma-Aldrich	T15760	Trizma® hydrochloride reagent grade, ≥99%, crystalline	1185-53-1	10KG	$1520	2024-03-01	Buy

Product number	Packaging	Price	Buy
T3253	50KG	$7730	Buy
T15760	500G	$113	Buy
T15760	1KG	$210	Buy
T15760	5KG	$895	Buy
T15760	10KG	$1520	Buy

TRIS hydrochloride Chemical Properties,Uses,Production

Description

Abbreviated as Tris HCl (TRIS hydrochloride), Tris (hydroxymethyl) aminomethane hydrochloride is an organic compound commonly utilized in buffer solutions such as TAE or TBE for electrophoresis gels. It boasts high solubility in water and offers utility within the pH range of 7.0-9.0.

Chemical Properties

TRIS hydrochloride is a white crystalline powder that has a pKa of 8.08 at 25°C and pH range of 7.0 to 9.0 that coincides with the physiological pH of most living organisms because of which it is commonly used as a component of buffer solutions in biology, biochemistry and molecular biology applications. Tris salts are also used for crystallization of proteins at various pH values. Neither Tris base nor Tris hydrochloride by itself provide adequate buffering capacity. Generally the two need to be mixed together to provide a buffer with pH between 7 and 9 to provide adequate buffering.

Uses

Tris Hydrochloride is a buffering medium for electrophoresis, molecular biology, and cell culture applications. It may be combined with Tris Base to simplify Tris buffer preparation. Tris Hydrochloride has been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs. In immunohistochemistry, Tris Hydrochloride has also been used with urea as a method for antigen retrieval.

Application

TRIS hydrochloride is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.

Preparation

The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.

General Description

TRIS hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Purification Methods

Crystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.]

TRIS hydrochloride synthesis

Synthesis of TRIS hydrochloride from Tris(hydroxymethyl)aminomethane

TRIS hydrochloride Preparation Products And Raw materials

Raw materials

Preparation Products

Glycine hydrochloride 2,2-DIMETHYL-1,3-DIOXAN-5-ONE

TRIS hydrochloride Suppliers

Global( 625)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Suzhou Genemill Biotechnology Co.,Ltd	+8615862502491	aimeegao@genemill.com	China	39	58
Yunbio Tech Co.,Ltd.	+86-010-60605551 +86-18046518538	yunbiochem@126.com	China	321	58
Hubei Xindesheng Material Technology Co., Ltd.	+8618971041571	vickyzhao@whdschem.com	China	174	58
Jiangsu Boquan Biotechnology Co., Ltd.	+86-18168774353	boquanshengwu003@boquansw.com	China	248	58
Hebei Chuanghai Biotechnology Co,.LTD	+86-13131129325	sales1@chuanghaibio.com	China	5892	58
Hebei Weibang Biotechnology Co., Ltd	+8615531157085	abby@weibangbio.com	China	8811	58
Hebei Mojin Biotechnology Co., Ltd	+86 13288715578 +8613288715578	sales@hbmojin.com	China	12839	58
Hebei Dangtong Import and export Co LTD	+86-13910575315 +86-13910575315	admin@hbdangtong.com	China	1000	58
Hebei Weibang Biotechnology Co., Ltd	+8617732866630	bess@weibangbio.com	China	18153	58
Henan Bao Enluo International TradeCo.，LTD	+86-17331933971 +86-17331933971	deasea125996@gmail.com	China	2472	58

Supplier	Advantage
Suzhou Genemill Biotechnology Co.,Ltd	58
Yunbio Tech Co.,Ltd.	58
Hubei Xindesheng Material Technology Co., Ltd.	58
Jiangsu Boquan Biotechnology Co., Ltd.	58
Hebei Chuanghai Biotechnology Co,.LTD	58
Hebei Weibang Biotechnology Co., Ltd	58
Hebei Mojin Biotechnology Co., Ltd	58
Hebei Dangtong Import and export Co LTD	58
Hebei Weibang Biotechnology Co., Ltd	58
Henan Bao Enluo International TradeCo.，LTD	58

Tris Hydrochloride: Preparation Method, Precautions, Advantages and Limitations
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May 10，2024

Tris Hydrochloride: Versatile Buffer Compound in Biological Research
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View Lastest Price from TRIS hydrochloride manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2024-11-25	TRIS hydrochloride 1185-53-1	US $980.00-750.00 / kg	1kg	99%	5000	HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD
2024-11-25	TRIS hydrochloride 1185-53-1	US $980.00-750.00 / kg	1kg	99%	5000	HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD
2024-11-22	TRIS HCl 1185-53-1	US $10.00 / kg	1kg	99%	20ton	Hebei Zhuanglai Chemical Trading Co.,Ltd

TRIS hydrochloride
1185-53-1
US $980.00-750.00 / kg
99%
HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD

TRIS hydrochloride
1185-53-1
US $980.00-750.00 / kg
99%
HEBEI SHENGSUAN CHEMICAL INDUSTRY CO.,LTD

TRIS HCl
1185-53-1
US $10.00 / kg
99%
Hebei Zhuanglai Chemical Trading Co.,Ltd

TRIS hydrochloride Spectrum

TRIS hydrochloride(1185-53-1)¹HNMR TRIS hydrochloride(1185-53-1)IR1 TRIS hydrochloride(1185-53-1)IR2 TRIS hydrochloride(1185-53-1)Raman

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T-BAG WITH GRANULATE FOR 5L TSB 3-propanediol,2-amino-2-(hydroxymethyl)-hydrochloride trizmahydrochloride(trishydrochloride) Tris(2-amino-2-hydroxymethyl)-1,3-propanediol, hydrochloride Tris(hydroxymethyl)aminomethane hydrochloride, extra pure, 99+% TrisHCl,mole-readyform,molecularbiologygrade TRISHYDROXYMETHYLAMINOMETHANE HYDROCHLORIDE BIO-REFINED TRIS(HYDROXYMETHYL)AMINOMETHANEHYDROCHLORIDE,BIOTECHGRADE TRIS(HYDROXYMETHYL)AMINOMETHANEHYDROCHLORIDE,CRYSTAL,REAGENT tris(hydroxymethyl)aminomethane hydrochliride(TRIS.HCL) 2-Amino-2-(hydroxymethyl)propan-1,3-diolhydrochlorid Tris-(hydroxymethyl)aminomethane hydrochloride (TRIS-Hydrochloride) TRIS HYDROCHLORIDE ULTRAPURE TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE >99.5% TRIS(HYDROXYMETHYL) AMINOMETHANE HYDORCHLORIDE TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE extrapure AR TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride Trizma hydrchloride TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE, 99+%, EXTRA PURE 3-propanediol hydrochloride TRIZMA(R) HYDROCHLORIDE, BIOPERFORMA TRIZMA(R) HYDROCHLORIDE, REAGENT GRA TRIZMA HYDROCHLORIDE, FOR LUMINE TRIZMA HYDROCHLORIDE, FOR MOLECU Tris, Hydrochloride, ULTROL TRIMETHYLOLAMINOMETHANE HYDROCHLORIDE TROMETHANE HCL TROMETHANE HYDROCHLORIDE TROMETAMOL HYDROCHLORIDE TRIS(HYDROXYMETHYL)AMINOMETHANE HCL BUFFER SUBST.PH 7.5* TRIS HCL 1 M STOCK SOLUTION PH 7.40 TRIZMA HCL, BIOTECHNOLOGY PERFORMANCE CE RTIFIED TRIS(HYDROXYMETHYL)AMINOMETHANE HCL 99% TRIS(HYDROXYMETHYL)AMINOMETHANE ULTRA PURE GRADE 99.5+% TRIS(HYDROXYMETHYL)AMINOMETHANE HYDROCHLORIDE（TRIS.HCL） 1MTrisHydrochlorideSolution,Ph9.0,Sterile Tris-HclTris(Hydroxymethyl)AminomethaneHydrochloride 1MTrisHydrochlorideSolution,Ph7.0 1MTrisHydrochlorideSolution,Ph10.0,Sterile Tris(Hydromethyl)AminomethaneHcl 1MTrisSolution,Ph8.0 Tris-HydrochlorideGr Tris(hydroxymethyl)aminomethane hydrochloride, 99+% Tris-HCl （Hydrochloride） Tris HCl (Tris hydrochloride) 2-AMINO-2-(HYDROXYMETHYL)-1,3-PROPANEDIOL, HYDROCHLORIDE SPECTRIS(R) HYDROCHLORIDE SPECTRIS(TM) HYDROCHLORIDE MOLE-READY-TRIS 1,3-Propanediol,2-amino-2-(hydroxymethyl)-,hydrochloride TRIS(HYDROXYMETHYL)AMINO-METHANE.HYDROCHLORIDE RES.GRADE TRIS(HYDROXYMETHYL)AMINOETHANE* HYDROCHLORIDE, MOLECULAR BIOLOGY GRADE Trizma hydrochloride,TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride Tris-HCl, molecular biology grade TRIZMA(R) HYDROCHLORIDE TRISMAT(TM) HYDROCHLORIDE TRISMAT(R) HYDROCHLORIDE TRIS HYDROCHLORIDE