EC 1.5.1.3
- CAS No.
- Chemical Name:
- EC 1.5.1.3
- Synonyms
- DHFR;EC 1.5.1.3;DIHYDROFOLATE REDUCTASE;DIHYDROFOLATE REDUCTASE HUMAN;TETRAHYDROFOLATE DEHYDROGENASE;TETRAHYDROFOLATE NADP+ OXIDOREDUCTASE;DHFR, Tetrahydrofolate NADP+ oxidoreductase
- CBNumber:
- CB4114482
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MFCD00130955
- MOL File:
- Mol file
| storage temp. | −20°C |
|---|---|
| FDA UNII | 5T4JAQ7QVD |
EC 1.5.1.3 Chemical Properties,Uses,Production
Uses
Human dihydrofolate reductase has been used in a study to investigate the stable expression of green fluorescent protein and the targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis. Human dihydrofolate reductase has also been used in a study to investigate the structural analysis of human dihydrofolate reductase as a binary complex.
General Description
Human DHFR is an 186 amino acid protein with an apparent molecular weight of 25 kDa. It is 30% homologous to the E. coli protein and up to 70% homologous to vertebrate proteins.
Biochem/physiol Actions
The human DHFR gene, as well as DHFR genes in other mammalian species, overcome the inhibitory effects of methotrexate by a mechanism of gene amplification or by amino-acid mutagenesis. Dihydroflate reductase (DHFR) catalyzes the NADPH dependent reduction of dihydrofolate (DHF) to tetrahydrofolate (THF) and, at a much lower rate, the conversion of folate to THF. The reaction product, THF, is an essential cofactor in the conversion of deoxyuridylate (dUMP) to deoxythymidylate (dTMP) by thymidylate synthetase. It is a key enzyme in thymidine synthesis. Therefore, DHFR is a critical enzyme in DNA synthesis and has become a target for drug development and cancer therapy. The variations between DHFR from different sources have enabled the development of species selective DHFR inhibitors, such as trimethoprim (antibacterial and antifungal), pyrimethamine (antiprotozoal), and methotrexate; MTX (antineoplastic, antipsoriatic, and anti-inflammatory).
EC 1.5.1.3 Preparation Products And Raw materials
Raw materials
Preparation Products
EC 1.5.1.3 Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Guangzhou Tomums Life Science Co., Ltd. | 020-31155029 18902330969 | sales@tomums.cn | China | 4698 | 58 |
| Shanghai Yaji Biological Technology Co., Ltd. | 021-34661275 15301693058 | yajikit@163.com | China | 9592 | 58 |
| Supplier | Advantage |
|---|---|
| Guangzhou Tomums Life Science Co., Ltd. | 58 |
| Shanghai Yaji Biological Technology Co., Ltd. | 58 |